蜂蜜黄酮类化合物检测方法建立及其在云南5种特色蜂蜜中的分布  

Establishment of Determination Method for Flavonoids in Honey and Distribution of Flavonoids in 5 Kinds of Characteristic Honey from Yunan

作　　者：梁铖[1] 卢焕仙[1] 刘宏程 张学文[1] 赵洪木[1] 宗德琴[1] 王艳辉[1] 

机构地区：[1]云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101 [2]农业部农产品质量安全风险评估实验室(昆明),云南昆明650223

出　　处：《食品科学》2013年第6期148-151,共4页Food Science

基　　金：国家蜂产业技术体系专项(CARS-45-SYZ17);云南省科技厅项目(2011BB012);云南省农业科学院蚕桑蜜蜂研究所青年创新基金项目(QC2010002)

摘　　要：建立蜂蜜中芦丁、杨梅酮、槲皮素、莰菲醇4种黄酮化合物含量的固相萃取-高效液相色谱检测方法。蜂蜜样品用pH2的盐酸溶液溶解、离心后,用Oasis HLB固相萃取柱吸附与净化,4种黄酮经VP-ODS色谱柱(150mm×4.6mm,5μm)分离,紫外检测器检测,检测波长270nm。流动相为甲醇-水(pH3.0)(60:40,V/V),流速0.7mL/min。检出限:芦丁、杨梅酮均为0.05mg/kg,槲皮素、莰菲醇均为0.04mg/kg;回收率:芦丁91%～92%、杨梅酮82%～85%、槲皮素84%～86%、莰菲醇86%～89%。分析云南省的5种特色蜂蜜样品,结果表明不同品种蜂蜜中4种黄酮类化合物含量差异显著,显示蜂蜜黄酮类化合物含量可作为鉴别蜂蜜品种的一个指标。A method for the determination of flavonoids in honey by SPE/HPLC was developed. The sample was extracted with acidified water （pH 2） and centrifuged, then enriched and cleaned-up by oasis HLB. The chromatographic separation was conducted on a VP-ODS （150 mm × 4.6 mm, 5 μm） column at the detection wavelength of 270 nm. The elution was completed by the mobile phase composed of methanol and water （pH 3） at the ratio of 60：40 （V/V） at the flow rate of 0.7 mL/min. The detection limits were 0.05 mg/kg for rutin and myricetin, and 0.04 mg/kg for quercetin and kaempferol, respectively. The recovery rates of rutin, myricetin, quercetin and kaempferol were 91%-92%, 82%-85%, 84%-86% and 86%-89%, respectively. Five kinds of characteristic honey from Yunnan were detected to reveal a significant difference in flavonoids. Therefore, ftavonoids may be as the markers for the identification of honey.

关 键 词：黄酮类化合物 特色蜂蜜 高效液相色谱 

分 类 号：S896.1[农业科学—特种经济动物饲养]