五指山小型猪内源性反转录病毒囊膜基因变异特征研究  

Analysis of variation feature for the env gene of porcine endogenous retrovirus from Wuzhishan miniature pigs

作　　者：张念[1,2,3] 马玉媛[2] 向思龙[2] 贾俊婷[2] 吴健敏[1,3] 章金刚[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]军事医学科学院野战输血研究所国家生物医学分析中心/病毒安全检测实验室,北京100850 [3]广西壮族自治区兽医研究所,广西南宁530001

出　　处：《中国预防兽医学报》2013年第3期248-250,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金资助项目(31071985);北京市自然科学基金资助项目(5122034)

摘　　要：为分析近交系连续3代次五指山小型猪内源性反转录病毒囊膜基因(PERV-env)变异特征,本研究采用PCR方法对该基因进行扩增、克隆和序列测定,将其与参考序列比对和遗传进化分析。序列分析显示:PERV-env核苷酸序列与参考序列之间的同源性为98.3%～99.3%,具有明显G→A突变,并且偏好发生于GA、GG。进一步分析表明,在PERV-env序列190 bp和1 875 bp位置均有G→A突变发生。PERV-env基因遗传进化树分析表明,五指山来源的PERV与国外小型猪PERV-A亲缘关系较近,但仍存在差异。本实验将有助于PERV分子特性的研究,为进一步开展异种移植中PERV的安全性评价奠定基础。To analyze the variation in env gene of porcine endogenous retrovirus （PERV）, PERV-env gene were cloned and sequenced from different generations of Wuzhishan miniature pigs （WZSPs） inbred. Compared with PERV reference sequence, the G to A mutation occurred in all the tested sequences, whose site preferences took place in 5＇-TC, CC （on the negative strand）. Two occurrence of stable G to A mutation sites in PERV-env sequences were found. Sequence analysis revealed that env gene shared 98.3% to 99.3% nucleotide identities with that of PERV reference strains. Phylogenetic analysis revealed that these tested sequences had high homology and close relationship with the PERV-A, but some differences still existed. This study will be helpful to investigations on the biological characteristics of PERV, and will contribute to the evaluation of viral safety in xenotransplantation.

关 键 词：近交系 五指山小型猪 猪内源性反转录病毒 囊膜基因 G→A突变 

分 类 号：S852.65[农业科学—基础兽医学]