miR-221在肝癌干细胞亚群中的差异表达  

Differential Expression of miR-221 in Subpopulations of Cancer Stem Cells Derived from Hepatocellular Carcinoma

作　　者：郭晓东[1,2] 杨敏亮 周艳贤[1] 刘树红[1] 皋月娟[2] 周光德[1] 赵雨来[1] 李进[1] 

机构地区：[1]军医进修学院 [2]解放军第302医院 [3]总后丰台综合仓库(62370部队)药材供应站

出　　处：《现代生物医学进展》2013年第3期465-467,563,共4页Progress in Modern Biomedicine

基　　金：国家"十二五"重大专项(2012ZX10002003-004-006)

摘　　要：目的:分离肝癌细胞系MHCC97中肝癌干细胞并分析肝癌细胞高表达miR-221在肝癌干细胞和非干细胞亚群中的表达差异情况,探讨miR-221表达水平与肝癌干细胞分化之间的关系。方法:利用流式细胞荧光激活分选法从肝癌细胞系MHCC97中分选出肝癌干细胞(hepatocareinoma stem cells,HSCs)和非干细胞(non-hepatocareinoma stem cells,non-HSCs)两个亚群。采用实时荧光定量RT-PCR(Real-time RT-PCR)检测miR-221在两个不同肝癌细胞亚群中的表达。结果:HSC亚群肝癌细胞仅占细胞总体的2.59%;HSC亚群细胞中miR-221的表达明显高于non-HSC亚群(P<0.01)。结论:miR-221在HSC亚群肝癌细胞中的明显高表达,提示miR-221可能在维持HSC亚群肝癌细胞的干细胞特性方面具有重要意义。通过调控肝癌干细胞中miR-221的表达,可以促进其分化成熟,从而为肝癌治疗提供新的思路。Objective： To isolate hepatocarcinoma stem cells from MHCC97 cell lines and investigate the expression characteristics of miR-221 in different subpopulations of MHCC97 cell line of human hepatocellular carcinoma （HCC）. Methods： By using flow cytometry and Hoechst 33342 dye eglux assay, hepatocareinoma stem cells （HSCs） and non-hepatocareinoma stem cells （non-HSCs） were isolated from MHCC97 human hepatoma cell. The expression levels of miR-221 in different subpopulations of MHCC97 cell line were measured by reverse transcription polymerase chain reaction （Realtime RTPCR）. Results： With the Hoechst 33342 extrusion assay. HSCs were found to account for 1.89% of the MHCC97 cell. The expression of miR-221 in HSCs was significantly higher than in non-HSCs （P〈0.01）. Conclusion： The significantly up-regulated expression of miR-221 in HSCs of MHCC97 cell line indicated that miR-221 may be involved in the maintenance of the stem cell property in hepatocareinoma stem cells. We could induce differentiation of HSCs through regulation the expression of miR-221, which will offer a possible therapeutic strategy for the treatment of hepatocellular carcinoma.

关 键 词：肝癌 MIR-221 肿瘤干细胞 

分 类 号：R735.7[医药卫生—肿瘤]