实验性胃溃疡大鼠前脑生长抑素神经元的变化  

Alterations of somatostatin neurons in the rat forebrain during experimental gastri culcer

作　　者：王建伟[1] 周济远[1] 郑慧娥[1] 杨天祝[2] 

机构地区：[1]张家口医学院组织胚胎学教研室,河北省张家口市075029 [2]河北医科大学基础医学研究所,河北省石家庄市050017

出　　处：《世界华人消化杂志》2000年第9期969-973,共5页World Chinese Journal of Digestology

基　　金：河北省教委科研基金课题;No.96103~~

摘　　要：目的观察大鼠实验性胃溃疡期间前脑生长抑素免疫反应(somatostatin,SS)和 SSmRNA 神经元的变化.方法用成年♂Wistar 大鼠38只,分为实验性胃溃疡组(EUG)、盐水对照组(SCG)和正常对照组(NCG).于术后1,4,10 d 和23 d 分批取脑,石蜡切片,用 Stemberger 免疫组化 PAP法显示弓状核后段水平前脑 SS 神经元;用地高辛标记反义SScRNA 探针和碱性磷酸酶标记抗地高辛抗体检测系统,显示SSmRNA 神经元.结果正常大鼠 SS 神经元广泛分布于前脑各核区,溃疡组术后1 d,大脑皮质纹状皮质区 SS 神经元减少,但可见大量 SS 强阳性纤维,SS 神经元数与盐水组相比无差异;在杏仁复合体和下丘脑各核区,溃疡组 SS 神经元数增多,与盐水组、正常组相比有差异[杏仁中央核(ce):EUG 57.1±3.0 vs SCG 41.7±1.2,杏仁外侧核(1a):EUG 56.3±1.2 vs SCG 49.3±2.7,杏仁皮质后核(pco):EUG 66.7±6.7 vs SCG 44.9±3.8,杏仁基外侧核(blp):EUG 65.0±8.3 vs SCG 34.2±3.0,下丘脑弓状核(ar):EUG 92.7±8.0 vs SCG 60.9±1.5,下丘脑腹内侧核(vmh):EUG 78.7±7.3 vs SCG 47.5±1.5,P<0.01;杏仁内侧核(me):EUG 77.3±6.0 vs NCG 41.7±2.2 andEUG vsSCG 49.8±2.1,下丘脑背外侧核(dmh):EUG 93.7±4.3 vsNCG 44.7±2.0 and EUG vs SCG 61.3±0.9,ce:EUG vsNCG 35.7±4.3,1a:EUG vs NCG 11.7±4.3,pco:EUG vsNCG 35.7±1.7,blp:EUG vs NCG 29.7±0.5,ar:EUG vsNCG 30.3±1.0,vmh:EUG vs NCG 30.7±4.3,P<0.001].术后4 d,溃疡组大脑皮质纹状皮质区、杏仁复合体和下丘脑各核区 SS 神经元密集,并在下丘脑弓状核及其周围可见较多"点样"阳性结构和串珠状 SS 阳性纤维;SS 神经元数明显增多,与盐水组、正常组相比均有差异(纹状皮质Ⅱ～Ⅲ:EUG 88.0±5.9 vs SCG 79.3±2.3.me:EUG 106.4±14.7 vs SCG 77.3±8.0 P<0.05;纹状皮质Ⅳ:EUG 83.5±3.8 vs SCG 50.7±2.0,纹状皮质Ⅴ～Ⅵ:EUG 79.5±4.4 vs SCG 70.0±1.3,ce:EUG 106.9±14.7 vs SCG 62.0±11.0,1a:EUG 83.5±6.6 vs SCG 60.0±5.3.pco:EUG 112.8±11.4 vs SCG 85.7±4.5,blp:EUG 90.1±8.4 vs SCG 60.0�AIM To observe the changes of SS and SSmRNA neurons in the rat forebrain during experimental gastric ulcer. METHODS Thirty-eight adult male Wistar rats were randomly divided into three groups,including experimental gastric ulcer group (EUG),saline control group (SCG) and normal control group (NCG).The animals were sacrificed on the 1st,4th,10th and 23rd days after operation of experimental gastric ulcer.The forebrains were removed and tissue blocks were embedded in paraffin and sectioned.Sternberger's PAP method was used to show SS neurons in the forebrain at the level of posterior part of the arcuate nucleus,SSmRNA neurons in the forebrain were expressed using in situ hybridization histochemistry with digoxigenin-labelled antiserum.SScRNA probe and anti-dig-alkaline phosphatase system. RESULTS SS neurons were widely distributed in the normal rat forebrain nuclei.On the 1st day after inducing ulcer,the SS neuron count was decreased,but varicose SS nerve fibers were dense and marked in striate cortex as compared with the normal control group,and there was no significant difference between experimental gastric ulcer group and saline control group.The SS cell counts in the amygdaloid complex and some nuclei of the hypothalamus were slightly increased on the 1st day in EUG.There was significant difference between EUG and SCG or NCG [central amygdaloid nucleus (ce):EUG 57.1 ±3.0 vs SCG 41.7±1.2,lateral amygdaloid nucleus (la): EUG 56.3±1.2 vs SCG 49.3±2.7,posterior cortical nucleus of the amygdala (pco):EUG 66.7±6.7 vs SCG 44.9±3.8,basolateral amygdaloid nucleus (blp).EUG 65.0±8.3 vs SCG 34.2±3.0,arcuate nucleus of the hypothalamus (ar):EUG 92.7±8.0 vs SCG 60.9±1.5, ventromedial hypothalamus nucleus (vmh):EUG 78.7± 7.3 vs SCG 47.5±1.5,P<0.01;medial amygdaloid nucleus (me):EUG 77.3±6.0 vs NCG 41.7±2.2 and EUG vs SCG 49.8±2.1,dorsomedial hypothalamus nucleus (dmh):EUG 93.7±4.3 vs NCG44.7±2.0 and EUG vs SCG 61.3±0.9,ce:EUG vs NCG 35.7±4.3,la:EUG vs NCG 11.7±4.3,pco:EUG vs NCG 35.7±1.7,blp:EUG vs NCG

关 键 词：胃溃疡 生长抑素 免疫组织化学 原位杂交 

分 类 号：R573.1[医药卫生—消化系统]