血清蛋白质组学无创诊断肝纤维化价值研究  

Serum proteomics:a non-invasive tool for the diagnosis of cirrhosis

作　　者：周迪[1,2] 赵长青[1,2,3,4,5] 徐品初[3] 刘新华[6] 徐列明[1,2,3,4,5] 

机构地区：[1]上海中医药大学附属曙光医院,上海201203 [2]上海中医药大学肝病研究所,上海201203 [3]肝肾疾病病证教育部重点实验室,上海201203 [4]国家中医药管理局慢性肝病虚损重点研究室,上海201203 [5]上海市中医临床重点实验室,上海201203 [6]上海中医药大学科技实验中心,上海201203

出　　处：《中国实用内科杂志》2013年第3期214-216,219,共4页Chinese Journal of Practical Internal Medicine

基　　金：国家重点基础研究发展计划(973计划)资助项目(2006CB504800);上海市教育委员会重点学科(第五期)建设项目(J50307);上海市高校E-研究院项目(E03008);上海市高校创新团队建设项目(第一期);国家中医药管理局中医肝胆病重点学科(2010sh)

摘　　要：目的探索血清蛋白质组学诊断肝纤维化的价值。方法选取2008年8月至2011年1月上海中医药大学附属曙光医院收治的103例乙型肝炎后肝纤维化患者,同时选取28例慢性乙型肝炎患者和29名健康志愿者的血液,分离血清,以纳米磁珠预处理,MALDI-TOF-MS检测,应用Autoflex Analysis软件对采集图谱进行分析。结果筛选出15个差异表达的蛋白质峰,建立乙型肝炎肝纤维化血清蛋白质诊断模型;筛选出2个差异表达的蛋白质峰,建立慢性乙型肝炎血清蛋白质诊断模型;筛选出各组差异表达的蛋白质峰,建立乙型肝炎肝纤维化Child-PughA、B、C级血清蛋白质模型。结论以血清蛋白质组建立的乙型肝炎肝纤维化和慢性乙型肝炎诊断模型,有助于临床鉴别诊断肝纤维化。Objective To investigate the significance of serum proteomics for the diagnosis of cirrhosisMethods Serum samples were obtained from 103 patients with hepatitis B-associated cirrhosis ,29 with chronic hepatitis B （CHB） and 29 healthy individuals who visited Affiliated Shuguang Hospital of Shanghai University of Traditional Chinese Medicine, be- tween August 2008 and January 2011. Serum isolated from the specimens were treated with nano magnetic beads （ Bruker Daltonics GmbH） for subsequent assay of serum proteomics with matrix-assisted laser desorption ionization time of flight mass spectrometry （MALDI-TOF-MS, Bruker Daltonics GmbI-I） by using the ClinProTools 2. 1 software for data capturing and processing. Results Fifteen culminations with differential protein expression were identified for establishment of the serum proteomics diagnostic model of hepatitis B-associated cirrhosis, whilst 2 culminations were screened for establishing the chronic hepatitis B diagnostic model. Furthermore, the culminations with differential protein expression in each group were employed to establish the diagnostic model of cirrhosis with Child-Pugh stages A, B and C. Conclusion The estab- lished diagnostic models for hepatitis B-associated cirrhosis and chronic hepatitis B based on serum proteomics may be help- ful to differential diagnosis of cirrhosis.

关 键 词：血清蛋白质组 乙型肝炎肝纤维化 慢性乙型肝炎 基质辅助激光解析离子化飞行时间质谱 

分 类 号：R575.2[医药卫生—消化系统]