利用HPLC法测定王草青贮饲料中的有机酸  

Determination of Organic Acids in King Grass Silage by HPLC

作　　者：张英[1,2,3] 周汉林[2,3] 刘国道[2,3] 王坚[1] 

机构地区：[1]海南大学农学院,海南儋州571737 [2]中国热带农业科学院热带作物品种资源研究所,海南儋州571737 [3]中国热带农业科学院热带草业与畜牧研究所,海南儋州571737

出　　处：《热带作物学报》2013年第2期377-381,共5页Chinese Journal of Tropical Crops

基　　金：中央级公益科研院所基本科研业务专项(No.PZS066);中国热带农业科学院院本级项目(No.1630032012049);国家现代牧草产业技术体系(No.CARS-35);农业部热带作物种质资源利用重点开放实验室开放基金:热带主要栽培牧草青贮发酵特性研究(No.KFKT-2011-10)

摘　　要：建立并优化了测定王草青贮饲料中乳酸(LA)、乙酸(AA)、丙酸(PA)、丁酸(BA)的高效液相色谱检测方法。采用20 mmol/L NaH2PO4/H3PO4(pH2.65)-甲醇二元梯度洗脱程序在高效液相色谱-紫外检测器上完成4种有机酸的定性定量分析。结果表明,4种有机酸在0.015～2.25 mg/mL范围内的线性关系较好,相关系数R2≥0.999 4,精密度、稳定性的RSD均小于5%,样品回收率在88.44%～114.51%,LA、AA、PA、BA的检出限分别是1.40、3.31、3.57、5.23μg/L。该方法简单、准确、重复性好,可用于王草青贮饲料中有机酸测定。A HPLC method was established and optimized to determinate lactic acid, acetic acid, propionic acid and butyric acid in king grass silage. Qualitative and quantitative analyses of the four organic acids were done by HPLC-UVD using gradient elution program with 20 mmol/L NaH2PO4/H3PO4 （pH2.65） and methanol. Results showed there was good linear relationships of the four organic acids. The correlation coefficients were above 0.999 4 in the range of 0.015-2.25 mg/mL, the precision and stability were less than 5%, the sample recovery was ranged in 88.44%-114.51%, the detection limit of lactic acid, acetic acid, propionie acid and butyric acid was 1.40, 3.31, 3.57, 5.23 μg/L, respectively. The method was simple, accurate and reproducible, and suitable for the determination of organic acids in king grass silage.

关 键 词：王草 青贮 高效液相色谱法 有机酸 

分 类 号：R927.2[医药卫生—药学]