三氧化二砷对NCI-H69肺癌细胞增殖和凋亡的影响  

PROLIFERATION INHIBITION AND APOPTOSIS INDUCED BY ARSENIC TRIOXIDE ON LUNG NEOPLASMS CELL LINE NCI-H69

作　　者：姚和瑞[1] 向燕群[1] 谢德荣[1] 陈岱佳[1] 曾宪平[1] 何剑峰[1] 

机构地区：[1]中山医科大学孙逸仙纪念医院,广州510120

出　　处：《中国现代医学杂志》2000年第6期3-4,6,共3页China Journal of Modern Medicine

摘　　要：目的 :探讨三氧化二砷 (AS2 O3)对肺癌细胞株NCI -H6 9的增殖抑制和诱导凋亡作用。方法 :MTT法观察不同浓度AS2 O3 作用不同时间后的NCI -H6 9生长的影响 ;增殖细胞核抗原 (PCNA)单抗LSAB免疫组化染色 -流式细胞仪检测PCNA阳性细胞百分比 ;FTIC -TUNEL法 -流式细胞仪检测细胞凋亡指数。结果 :随AS2 O3 浓度增加和作用时间延长 ,NCI -H6 9肺癌细胞的细胞活度渐下降。随AS2 O3 浓度增加 ,PCNA阳性细胞百分比逐渐下降 ,而凋亡指数逐渐升高。结论 :三氧化二砷可明显抑制肺癌细胞株NCI -H6 9的生长 ,并能诱导细胞凋亡 。Objective: To observe the effect of proliferation inhibition and apoptosis indution of Arsenic Trioxide (AS2O3) on lung neoplasms cell line NCI-H69. Methods: MTT assay was used to observe the proliferation status. Flow cytometry was used to measure the percentage of proliferation cell nuclear antigen (PCNA) positive cell and apoptosis index. Results: AS2O3 could significantly inhibit the growth of NCI-H69 cell, this effect was dose and time dependent. The percentage of PCNA positive cell was decreased gradually when concentration of AS2O3 was increased. The apoptosis index increased by the increasing of concentration of AS2O3. Conclusions: Arsenic Trioxide could inhibit the proliferation activity and induce apoptosis of lung neoplasms cell line NCI-H69. It may have potential value in the treatment of lung cancer.

关 键 词：肺肿瘤 三氧化二砷 细胞凋亡 增殖细胞核抗原 

分 类 号：R734.205[医药卫生—肿瘤]