利用双水相萃取技术分离纯化尿激酶  

Separation and purification of urokinase by aqueous two-phase extraction

作　　者：李兵[1] 

机构地区：[1]邢台医学高等专科学校,河北邢台054000

出　　处：《生物学通报》2013年第3期49-51,共3页Bulletin of Biology

摘　　要：通过研究双水相萃取系统的各种影响因素:乙醇/(NH4)2SO4的组成比例、pH值、无机盐的加入、粗酶的浓度等,探索以乙醇/(NH4)2SO4组成的双水相萃取体系纯化尿激酶的最佳条件。建立以乙醇/(NH4)2SO4组成的双水相萃取体系分离纯化尿激酶的新途径。结果表明:双水相萃取系统冰乙醇浓度为65%,(NH4)2SO4浓度为10.0%,pH8.0,酶加入量为30%,且不加入任何其他无机盐的条件下,尿激酶的纯化倍数可达到9.2倍,回收率最高达92%。An aqueous two-phase extraction system using ethanol/（ NH4 ）2 SO4 to separate urokinase was established. Factors of ethanol/（ NH4 ）2S04 ratio, pH, inorganic salt and crude enzyme concentration influencing the purification were investigated . The results showed that 65% cold ethanol, 10% （NH4）2SO4,pH8. 0, crude enzyme 30%. Under the optimal conditions, no inorganic salt was added. Purification urokinase was up to 9 times ,and the recovery was 92%.

关 键 词：双水相 尿激酶 萃取 纯化 

分 类 号：Q814.4[生物学—生物工程]