山麦胶囊对血管内皮细胞的保护作用研究  

The Researches about Protection of Shanmai Capsules on Vascular Endothelial Cells

作　　者：秦秋华[1] 周燕[1] 蒋伟哲[1] 施晓霞[1] 龙凤鸣 韦秀芝 

机构地区：[1]广西医科大学药学院,广西南宁530021 [2]广西河池市第一人民医院,546300

出　　处：《时珍国医国药》2013年第1期63-65,共3页Lishizhen Medicine and Materia Medica Research

基　　金：广西自然科学基金(桂科自0991264)

摘　　要：目的观察山麦胶囊对人脐静脉内皮细胞(HUVECs)的保护作用。方法用不同浓度的山麦胶囊含药血清培养HUVECs 24h,测定培养液中一氧化氮合酶(eNOS)活性和一氧化氮(NO)含量;用肿瘤坏死因子-α(TNF-α)诱导损伤HUVECs,用ELISA法测定损伤模型组和含药血清组培养液中血管细胞粘附分子-1(VCAM-1)和细胞间粘附分子-1(ICAM-1)的含量。结果含药血清组HUVECs中eNOS活性和NO含量显著升高(P<0.05);与模型组相比,含药血清组细胞形态无明显损伤,同时VCAM-1和ICAM-1的含量水平明显下降(P<0.05)。结论山麦胶囊能够增加HUVECs中eNOS的活性,使NO生成增多,抑制VCAM-1和ICAM-1生成,对血管内皮细胞具有保护作用。Objective To observe the protection of Shanmai capsules on human umbilical vein endothelial ceils （HUVECs）. Methods Routine culture HUVECs with different concentrations of Shanmai capsules containing serum 24h, measured the level of nitric oxide（NO） and endothelial nitric oxide synthase （eNOS） in cell culture fluid. Tumor necrosis factor-α （TNF -α） was used to induce inflammatory responses in HUVECs, then HUVECs were co - incubated with different concentrations of Shanmai capsules containing serum, measured the level of vascular cellular adhesive molecular - 1 （ VCAM - 1 ） and intercellular cellular adhesive molecules - 1 （ ICAM - 1 ） in cell culture fluid by ELISA. Results Shanmai capsules containing serum not only in- creased activities of eNOS and content of NO in HUVECs obviously（P 〈0.05）, but also reduced the content of VCAM - 1 and ICAM -1 at the same time（P 〈0.05）. Besides, compare with model control group, the shape of HUVECs in Shanmai capsules containing serum group had not great changed. Conclusion Shanmai capsules could protect vascular endothelial cells by inhibition of the release of VCAM - 1 and ICAM - 1. And the mechanism may be related to the increase of eNOS activities and NO content.

关 键 词：山麦胶囊 脐静脉内皮细胞 肿瘤坏死因子-Α 脐静脉内皮细胞 细胞间粘附分子 

分 类 号：R285.5[医药卫生—中药学]