燕窝酶解产物中多肽的液相色谱质谱分析  

Peptides analysis in digested edible bird′s nest by HPLC-MS

作　　者：刘琳[1] 李修乐[1] 高建萍[2] 孔英俊[2] 王明林[1] 张贵锋[2] 苏志国[2] 

机构地区：[1]山东农业大学食品科学与工程学院,山东泰安271018 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190

出　　处：《中国中药杂志》2013年第5期714-719,共6页China Journal of Chinese Materia Medica

基　　金：国家高技术研究发展计划(863)项目(2010AA023005);北京市自然科学基金项目(7102108)

摘　　要：目的:针对燕窝中蛋白质糖基化程度不均一和相对分子质量范围不稳定,以及由此导致的蛋白质难以分离、分析和鉴别的问题,研究1种燕窝中蛋白质和多肽的分析方法。方法:利用分步法从燕窝中提取了蛋白质组分,采用糖苷酶和蛋白酶对蛋白质组分进行了处理,采用生物质谱技术对蛋白质降解物进行了分析,并对其中的多肽进行了序列分析。结果:燕窝中的蛋白提取率为79.7%,采用多级质谱技术从提取蛋白质的糖苷酶和胰蛋白酶处理后的混合物中检测出20种多肽。结论:这些多肽的序列与已有蛋白质数据库中不同种类蛋白的局部序列相似,为富含糖蛋白的动物药成分研究提供了技术参考。Edible bird＇s nest contains lots of glycoproteins. The glycosylation inhomogeneity for glycoprotein often results in wide range of molecular weight and the difficulty for protein separation and charaterization. In this paper, proteins in the edible bird＇s nest were extracted using multiple extractions, and then digested by PNgase F and trypsin. The digest mixture was separated with HPLC, and peptides were identified based on MS/MS data searching. The results indicated that the extracted proteins were amount to 79.7% of total protein in the edible bird＇s nest. More than 20 species of peptides in the digested mixture were identified. The se- quences of these peptides showed similarity with some proteins from Swiss-prot. The research indicated that deglycosylation, tryptic di- gestion coupled with HPLC-MS/MS is a proper strategy for characterization of proteins in the edible bird＇s nest.

关 键 词：燕窝 糖蛋白 酶解 质谱分析 序列识别 

分 类 号：R284[医药卫生—中药学]