高效阿特拉津降解菌株DNS10降解条件优化  

Optimization for degradation conditions of atrazine-degrading strain DNS10

作　　者：张庆媛[1] 葛世杰[1] 姜昭[1] 王志刚[1] 张颖[1] 

机构地区：[1]东北农业大学资源与环境学院,哈尔滨150030

出　　处：《环境工程学报》2013年第3期1169-1174,共6页Chinese Journal of Environmental Engineering

基　　金：哈尔滨市科技创新人才研究专项基金(2012PFXXN013);国家新世纪优秀人才计划项目(NCET-10-0145);黑龙江省杰出青年学者科学基金(JC201006);黑龙江省新世纪优秀人才计划项目(1155-NCET-006);"十二五"农村领域国家科技计划(2011BAD04B02-1);国家自然科学基金资助项目(30970525)

摘　　要：从长期施用阿特拉津的寒地黑土耕层(0～10 cm)土壤中筛选到一株能以除草剂阿特拉津为氮源生长的降解菌株,结合16S rRNA序列分析结果,将该菌株命名为Arthrobacter sp.DNS10。在接种量为108CFU/mL的条件下,菌株DNS10在24 h内对100 mg/L阿特拉津的降解率为99.41%。单因子实验结果表明,菌株DNS10适宜生长和降解的条件范围是:温度25～35℃,pH值5.0～8.0,培养液盐度0.1%～2%,对阿特拉津最大耐受浓度可达1 200 mg/L。正交实验法进一步表明,该菌株保持较好生长及降解能力的最优方案是温度30℃,pH值7.5,培养液盐度0.5%。影响其降解能力的环境因素的主次顺序依次是:温度>盐度>pH值。An atrazine-degrading strain, which grew with atrazine as the nitrogen source, was isolated from the top of black soil （0 - 10 em） in cold area of long-term applying atrazine. The strain was designated as Arthrobacter sp. DNS10 according to the sequence of 16S rRNA. It is capable of removing 100 mg/L atrazine in medium about 99.41% within 24 h with 10s CFU/mL inoculation. The results of single factor analysis show the optimal conditions for growth and degradation ability of DNSIO. The range of temperature is 25 - 35℃ , pH is 5.0 -8.0, medium salinity is 0. 1% -2% and the maximum resistance concentration of atrazine is 1 200 mg/L. Furthermore, based on orthogonal experiments, the optimal combination scheme for the growth and degradation ability of DNS10 is that temperature is 30℃ , pH is 7.5 and medium salinity is 0.5%. The primary and secondary order of environmental factors that influence the degradation ability of DNS10 is that： temperature 〉 salinity 〉 pH

关 键 词：阿特拉津(AT) 菌株DNS10 降解能力 环境因素 

分 类 号：X172[环境科学与工程—环境科学]