应用扫描隧道显微镜技术观察针对HBsAg基因转录物的核酶的二级结构  

OBSERVATION OF THE SECONDARY STRUCTURE OF THE RIBOZYME DESIGNED TO THE TRANSCRIPT OF HBsAg GENE BY SCANNING TUNNELLING MICROSCOPY(STM)

作　　者：王平[1] 郭军[2] 白春礼[2] 侯云德[3] 

机构地区：[1]中国预防医学科学院病毒学研究所病毒基因工程国家重点实验室 [2]中国科学院化学研究所 [3]中国预防医学科学院病毒学研究所病毒基国工程国家重点实验室

出　　处：《病毒学报》1991年第4期369-371,共3页Chinese Journal of Virology

摘　　要：核酶(Ribozyme)是具有催化RNA切割反应功能的RNA,它可以特异性地切割RN-A。最近,核酶的一级结构已经被证实,其与底物结合时所形成的二级结构如锤头状(Hammerhead)、发夹型(Hairpin)结构,亦得到初步证实。因此,能够设计并人工合成核酶,通过特异性破坏RNA,阻断其功能,从而有效地阻止病毒的复制和繁殖。这是近年来继反义RNA之后发现的又一个抑制基因表达的有力工具,开辟了分子生物学的一个新领域。Ribozyme is a kind of RXA which can catalyze cleavage of the sequence-specific RNA. However, there have been no direct evidence for Ribozyme's spatial structure. We applied the technology of Scanning Tunnelling Microscopy ( STM ) to intend to get some direct information, about the spatial structure of the hammerhead-structure Ribozyme,which was artificial synthesized and designed to the transcript of HBsAg gene. The Ribozyme's molecular length and its greatest width was 286.8 A and 20.0 A. The Ribozyme molecules stretched out linearly on graphite surface and were not as expected to form a hairpin or other complicated structure.

关 键 词：核酶 HBSAG 基因转录 STM 

分 类 号：Q939.4[生物学—微生物学]