携带TSLC1基因的双靶向溶瘤腺病毒联合阿霉素对SMMC-7721肝癌细胞的杀伤作用  

Killing Effect to Hepatoma Cell SMMC-7721 by Dual-regulated Oncolytic Adenovirus-Mediated TSLC1 Combined with Adriamycin

作　　者：郭科妮[1] 卓玲燕 刘涛[1] 李晓艳[1] 李阿荣[1] 王毅刚[1] 

机构地区：[1]浙江理工大学生命科学学院新元医学与生物技术研究所,杭州310018

出　　处：《中国细胞生物学学报》2013年第3期282-289,共8页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:81272687);浙江理工大学科研启动基金(批准号:1016845-Y;1016834-Y)资助的课题~~

摘　　要：研究携带TSLC1基因的溶瘤腺病毒(Ad.sp-E1A(24)-TSLC1)联合化疗药物阿霉素(adriamycin,ADM)对SMMC-7721肝癌细胞的体外杀伤作用。实时定量PCR检测TSLC1在不同肝细胞中的表达情况;用荧光显微镜观察阿霉素、Ad.sp-E1A(24)-TSLC1单独作用和两者联合作用的细胞形态学的变化;采用MTT法、结晶紫染色、Hoechst33342染色检测各处理组细胞的存活率和凋亡水平情况;通过Western blot检测TSLC1和E1A蛋白水平的表达。结果表明,联合应用Ad.sp-E1A(24)-TSLC1和阿霉素的细胞凋亡现象比单独作用的效果显著,表明阿霉素能够增强携带TSLC1基因的溶瘤腺病毒对肝癌细胞SMMC-7721的杀伤作用,为肝癌治疗的临床应用奠定一定基础。The study explores the combinational killing effects of dual-regulated oncolytic adenovirus- mediated TSLC1 （Ad.sp-E1A^A24）-TSLC1） and adriamycin （ADM） against hepatoma cell SMMC-7721 in vitro. The method of RT-PCR was performed to test the expression of TSLC1 at the level of transcription. The morphological change was assessed by fluorescence microscope. MTT assay, crystal violet assay and Hoechst33342 staining were used to determine the growth inhibition effect and cytopathic effect of single or combination therapy on hepatoma cell SMMC-7721. The expression level of TSLC1, E1A protein was assayed by Western blot. These results demonstrated that combination therapy of Ad.sp-E 1A（a24）-TSLC 1 and ADM induces apoptosis characteristics more significantly than either single treatment, and low doses of ADM enhanced the killing effects of dual-regulated oncolytic adenovirus-mediated TSLC1.

关 键 词：TSLC1 溶瘤腺病毒 阿霉素 SMMC 7721 

分 类 号：R735.7[医药卫生—肿瘤]