钇(Ⅲ)-钙黄绿素-吐温80体系荧光光度法测定痕量钇  

Determination of trace yttrium by fluorescence spectrophotometry with Y(Ⅲ)-calcein-Tween 80 system

作　　者：高英杰[1] 柳玉英[1] 陈娟平[1] 

机构地区：[1]山东理工大学化学工程学院,山东淄博255049

出　　处：《冶金分析》2012年第6期52-55,共4页Metallurgical Analysis

基　　金：山东自然科学基金(Y2008B26)

摘　　要：在硼酸-硼砂缓冲溶液介质中,表面活性剂吐温80的存在下,痕量钇(Ⅲ)离子能显著提高钙黄绿素的荧光强度,据此建立了一种测定痕量钇(Ⅲ)的新体系。考察了荧光体系的光谱特征,研究了试剂用量、酸度、温度、时间及共存离子对测定的影响。结果表明,体系的最大激发波长(λex)和发射波长(λem)分别为492和512nm,钇(Ⅲ)的含量在0.04～2.0μg/mL范围内与△F呈良好的线性关系,其回归方程为△F=2.384ρ+3.264 8,相关系数为0.996 1,检出限为0.023μg/mL。此法用于醋酸铈中钇的测定,测定结果与电感耦合原子发射光谱法一致,相对标准偏差(RSD,n=6)为1.4%～2.1%。In the medium of boric acidborax buffer solution and with the presence of surfactant Tween 80, trace yttrium （Ⅲ） ions could significantly increase the fluorescence intensity of calcein. Consequent ly, a novel system for determination of trace yttrium aid was proposed. The spectral characteristic of fluorescence system was investigated, and such influence factors on the reaction were discussed as the influence of reagent amount, acidity, temperature, time and coexisting ions. Experiments results indi cated that the maximum excitation （λex） and emission wavelengths （kom） of the system were 492 and 512 nm respectively. The yttrium（Ⅲ）content in the range of 0.042.0μg/mL had excellent linear rela tionship with/kF, and its regression equation was AF=2. 3849＋3. 264 8 with correlation coefficient of 0. 996 1. Detection limit of this method was 0. 023/lg/mL. The method was applied to the determi nation of yttrium in cerium acetate. The results were consistent with those obtained by inductively coupled plasma atomic emission spectrometry, and the relative standard deviations （RSDs, n= 6）were 1.4%2.1%.

关 键 词：荧光光度法 钇 钙黄绿素 吐温80 

分 类 号：O657.32[理学—分析化学]