基于磁性纳米颗粒微阵列技术的MT-2A基因启动子单核苷酸多态性分析  

Analysis of Single Nucleotide Polymorphism in the Promoter of MT-2A Gene by Magnetic Nanoparticle-Based Microarray Technology

作　　者：荣高翔[1] 傅娟[1] 

机构地区：[1]湖南工业大学绿色包装与生物纳米技术应用重点实验室,湖南株洲412007

出　　处：《湖南工业大学学报》2012年第6期100-104,共5页Journal of Hunan University of Technology

基　　金：湖南省教育厅基金资助项目(10C0629);湖南省自然科学基金资助项目(11JJ5011)

摘　　要：根据高通量检测手段对冶炼作业人群中金属硫蛋白MT-2A基因启动子核心区域进行单核苷酸多态性(SNP)分析。通过磁性纳米颗粒微阵列技术对冶炼作业工人(288例)的MT-2A基因启动子位点进行高通量SNP检测,并进行测序验证。结果表明:该冶炼作业人群中MT-2A基因启动子核心区域的3种基因型(AA,AG和GG)频率分别为87.4%,11.8%和0.8%,等位基因(A和G)频率分别为93.2%和6.8%。该人群MT-2A基因型与等位基因频率均符合Hardy-Weinberg遗传平衡检测(p>0.05)。最后,利用直接测序法对PCR产物进行测序,所得结果与SNP分析结果一致。By high throughput detection means, the single nucleotide polymorphisms （SNPS） analysis is conducted on metallothionein MT-2A gene promoter core region of metallurgy operation population. The MT-2A gene promoter sites of metallurgy workers （288 cases） are carded on high throughput SNP detection by magnetic nanoparticles microarray technology, and the sequencing test is also made. The results show that three genotypes （AA, AG and GG） frequency of the MT-2A gene promoter core region in the workers are 87.4%, 11.8% and 0.8%, and alleles （A and G） frequency are 93.2% and 6.8%, respectively. The MT-2A genotype and allele frequencies are consistent with Hardy-Weinberg equilibrium （p〉0.05）. Finally applies the direct sequencing method for PCR product sequencing test, and the obtained results are consistent with the result of SNP analysis.

关 键 词：磁性纳米颗粒 微阵列技术 金属硫蛋白 单核苷酸多态性 

分 类 号：Q524.3[生物学—生物化学]