血浆miR-206在结直肠癌中的表达及意义  

Expression of plasma miR-206 expression in colorectal cancer and significance

作　　者：徐伶玲[1] 马莹[1] 范慧娟[1] 吴静[1] 安广宇[1] 

机构地区：[1]首都医科大学附属北京世纪坛医院消化内科,北京100038

出　　处：《中华实验外科杂志》2013年第3期444-447,共4页Chinese Journal of Experimental Surgery

基　　金：国家高技术研究发展计划（863）计划资助项目（2012AA02A506）;北京市卫生焉尊高层次卫生技术人才培养计划项目

摘　　要：目的筛选结直肠癌患者血浆中差异表达的微小RNA（miRNA）。方法通过TaqMan^MicroRNAArray检测7例结直肠癌患者和6例健康体检者血浆中差异表达的miRNA，应用实时荧光定量聚合酶链反应（Real—timePCR）在29例结直肠癌患者和20例健康体检者中进行验证，并分析血浆差异表达的miRNA在结直肠癌中的价值。结果初步筛选得到16个结直肠癌血浆差异表达的miRNA，Real—timePCR验证得出miR-125b、miR-375、miR-150、miR-206与初筛结果。致且差异有统计学意义（P〈0．05）。血浆miR-206表达水平对区分结直肠癌患者与健康体检者意义更大，miR-206每增加1个单位，发生结直肠肿瘤风险增加为原来的0．29倍[比值比（OR）=0．29，95％可信区间（CI）：0．12～0．67]，在肿瘤早期就会出现表达水平的明显下降（P〈0．01）。结论血浆miR-206与结直肠癌的发展密切相关。Objective To screen out the plasma miRNA which may be differentially expressed in colorectal cancer. Methods Differential expression of plasma miRNA was detected by using TaqMan~ MieroRNA Array from 7 patients with colorectal cancer and 6 healthy volunteers. Th~ results were validated in plasma samples of 29 patients with colorectal patients and 20 healthy volunteers by real-time polymerase chain reaction （Real-time PCR）. Results Sixteen plasma-specific miRNAs were identified from patients with colorectal cancer, miR-125b, miR-375, miR-150 and miR-206 validated by real-time PCR were con- sistent with TaqMan~ MicroRNA Array results and had statistical significant difference （P 〈0. 05）. Fur- thermore, the expression of plasma miR-206 was more meaningful to distinguish the patients with coloreetal cancer from healthy persons. When plasma miR-206 was increased by one unit, the occurrence risk of colo- rectal cancer was 0. 29 [ odds ratio（OR） = 0. 29,95% confidence interval（CI） ：0. 12-0. 67 ] and its expres- sion level was decreased in the early stage of tumor （ P 〈 0. 01 ）. Conclusion Plasma miR-206 was expec- ted to be as a non-invasive biomarkerfor early orediction of colorectal cancer.

关 键 词：结直肠肿瘤 微小RNA 血浆 肿瘤标志物 

分 类 号：R735[医药卫生—肿瘤]