白细胞介素-22对缺氧条件下肠上皮屏障的保护作用及其机制  

Preventive effect of interleukin-22 on epithelial barrier function of T84 cells under hypoxia and pos-sible mechanism

作　　者：杨学超[1] 杨松巍[1] 杨光[1] 孙力华[1] 杨桦[1] 

机构地区：[1]第三军医大学新桥医院普通外科,重庆400037

出　　处：《中华实验外科杂志》2013年第3期476-479,共4页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金重大国际合作项目（81020108023）;国家自然科学基金资助项目（30973113、81272078）;重庆市科委国际合作重点项目（201110008）

摘　　要：目的观察白细胞介素-22（IL-22）对缺氧肠上皮T84细胞屏障功能的影响，探讨其分子机制。方法对T84细胞进行2％02缺氧6h（Hx组）及100μg／L重组IL-22（Hx＋rlL-22）处理，分别检测跨上皮电阻（TER），IL-22受体A1（IL-22RAl）及紧密连接蛋白：ZO-1、Occludin、Claudin-1及Claudin-4的mRNA与蛋白表达。结果缺氧引起IL-22RAl的mRNA及蛋白表达较常氧分别下降30．0％及63．0％，并引起TER降低54．7％，经rlL-22预处理使得TER较Hx升高43．8％。缺氧处理使ZO．1、Occludin、Claudin．1及Claudin-4表达明显降低，而rlL-22预处理则可缓解该现象（mRNA及蛋白表达较Hx组分别升高：44．3％、36．4％、77．6％、61．2％及109．3％、77，6％、153．3％、79．6％，P〈0．01）。结论IL-22与其受体作用后可以维持肠屏障功能，稳定渗透性，调控紧密连接蛋白的表达。Objective To investigate the effect of interleukin-22 （IL-22） on intestinal barrier function of T84 cells under hypoxia and the molecular mechanism. Methods T84 cells were randomly di- vided into 3 groups： the normoxia group （Nx） , the hypoxia group （Hx, 2% 02 for 6 h） and hypoxia plus 100 μg/L rIL-22 group （ Hx ＋ rIL-22 ）. Transepithelial electrical resistance （TER） was determined, and the protein and mRNA expression of IL-22 receptor A1 （ IL-22RA1 ） was detected, zonula occludens-1 （ ZO-1 ） , Occludin, Claudin-1 and Claudin-4 expression was examined by using reverse transcription-poly- merase chain reaction （RT-PCR） and Western blotting. Results Both mRNA and protein expression lev- els of IL-22RA1 were significantly reduced by 30.0% and 63.0% respectively in Hx group as compared with Nx group （P 〈0. 01 ）. The TER value in Hx group was decreased by 54. 7% as compared with Nx group （P 〈 0. 01 ）, which could be reversed through the pretreatment of rIL-22 （P 〈 0.01 ）. The expres- sion levels of ZO-1, Occludin, Claudin-1 and Claudin-4 in Hx group were lower than in Nx group （ P 〈0.01 ）, but pretreatment with rlL-22 could attenulate the decreased expression of TJs under hypoxia （P 〈 0.01 ）. Conclusion After binding to its receptor complex, IL-22 could maintain the epithelial barrier function through regulating the expression of TJs.

关 键 词：白细胞介素-22 肠上皮屏障 跨上皮电阻 紧密连接蛋白 

分 类 号：R57[医药卫生—消化系统]