大鼠肝脏低温灌注和复流模型改进及B7表达的研究  

Improvement of liver hypothermic irrigation and reperfusion model and expression of B7 in rats

作　　者：米曰堂[1] 李蕊[1] 马静[2] 翟慧[2] 

机构地区：[1]山东省聊城市人民医院普外科,山东聊城252000 [2]山东省聊城市人民医院手术室,山东聊城252000

出　　处：《中华实验外科杂志》2013年第3期511-513,共3页Chinese Journal of Experimental Surgery

摘　　要：目的探讨共刺激分子B7—1和B7-2在大鼠肝脏低温灌注和复流模型中的表达及其免疫学意义。方法将30只大鼠随机分为3组：A组（低温灌注20min再复流24h）、B组（低温灌注30min再复流24h）、C组（假手术组）。分别取模型之肝脏，应用逆转录聚合酶链反应（PT-PCR）检测B7．1和B7-2在3组肝脏组织中mRNA表达。结果B7—1和B7-2mRNA在C组以极低水平表达（0．226±0．053，0．339±0．049），而在A组、B组表达却明显增多（P〈0．01），且B组明显高于A组（B7。1：0．475±0．079比0．3674-0．052，B7-2：0．666±0．039比0．595±0．050；P〈0．01）。结论低温灌注和复流之肝脏通过上调B7—1和B7-2的表达来增加肝脏的免疫原性。Objective To elucidate the expression of costimnlatory molecule B7-1 and B7-2 in hv- er hypothermic irrigation and reperfusion model of rats and its significance. Methods Thirty rats were ran- domly divided into three groups ： group A, hypothermic irrigation for 20 min ; group B, hypothermic irriga- tion for 30 min; and group C, sham operation. The expression of B7-1 and B7-2 mRNA in the livers was detected by using real-time reverse transcription polymerase chain reaction （RT-PCR）. Results The B7-1 and B7-2 expression levels were at very low ievels in the liver tissues of group C （0. 226 ~ O. 053, and 0. 339 _＋0. 049）, but the mRNA expression levels of B7-1 and B7-2 were significantly enhanced in group A or group B （P〈0. 01）. In addition, both B7-1 and B7-2 levels were higher in group B than in group A （B7-1 ： 0.475±0. 079 vs O. 367±0. 052; B7-2：0. 666 ＋0. 039 vs O. 595±0. 050;P 〈 0. 01 ）. Conclu- sion Hypothermic irrigation and reperfusion may increase the immunogenticity of the liver by upregnlating the costimulatory molecules B7-1 and 137-2.

关 键 词：肝脏 低温灌注 B7表达 

分 类 号：R575[医药卫生—消化系统] R-332[医药卫生—内科学]