人膀胱移行细胞癌上皮间质转化与核转录因子叉头框蛋白Q1的关系  

Correlation between transcription factor forkhead box protein Q1 and epithelial-mesenchymal transition in human bladder transitional cell carcinoma

作　　者：朱智能[1] 朱朝辉[1] 庞自力[1] 兰东阳[1] 王海鹏[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院泌尿外科,武汉430022

出　　处：《中华实验外科杂志》2013年第3期567-570,共4页Chinese Journal of Experimental Surgery

摘　　要：目的探讨膀胱移行细胞癌（BTCC）上皮间质转化（EMT）与核转录因子叉头框蛋白Ql（FOXQl）的相关性，以及FOXQl与膀胱癌临床病理特征之间的关系。方法选用膀胱移行细胞癌手术切除标本65例，每例标本均选用癌组织中心、癌旁组织及其远端正常黏膜组织对照。采用免疫组织化学、逆转录-聚合酶链反应（RT—PCR）和Westernblot技术检测各例标本中转化生长因子（TGF）．B1、核转录因子FOXQl及上皮问质标志物E一钙黏蛋白（E．cadhefin）和波形蛋白（Vime^in）的表达，并分析与临床病理因素之间的关系。结果（1）FOXQl在BTCC组织中的表达高于正常黏膜组织（89．2％比13．8％，P〈0．05），其在肌层浸润性癌中的表达高于浅表性癌（97．3％比78．6％），在有淋巴结转移癌中的表达明显高于无淋巴结转移癌（100．O％比86．8％）；TGF．B1在BTCC组织中的表达高于正常黏膜（76．9％比26．2％，P〈0．05）；E—cadhefin在BTCC组织中的表达低于正常黏膜（27．7％比89．2％，P〈0．05）；Vime^in在BTCC组织中的表达高于正常黏膜（83．1％比20．0％，P〈0．05）。（2）FOXQl与E—cadhefin表达呈负相关，与TGF-61、Vimentin表达呈正相关，是EMT的正向调控因子。结论核转录因子FOXQl与膀胱移行细胞癌分化和转移有关，可能通过调榨EMT来促进膀胱移行细胞癌的侵袭转移。Objective To investigate the relationship between transcription factor forkhead box protein Q1 （FOXQ1） and epithelial mesenchymal transition （EMT） in bladder transitional cell carcinoma （BTCC）, and the association between FOXQ1 and clinicopathologic features. Methods Sixty-five BTCC specimens surgically reseeted were selected. For each specimen, the cancerous tissue, peri-cancerous tis- sue and the remote normal mucosa were analyzed and compared. The expression of FOXQ1, transforming growth factor （TGF）-131, E-cadherin and Vimentin genes in resected cancer tissues was detected by using reverse transcription-polymerase chain reaction （RT-PCR）, Western blotting and immunohistochemistry, and their association with clinicopathologic data was analyzed. Results （ I ） The expression rate of FOXQ1 was significantly higher in BTCC tissues than in normal tissues （89.2% vs 13.8% ,P 〈0.05）, and that in deeply infiltrating group was significantly higher than that ir~ superficially infiltrating group （ 97.3% vs 78.6% ,P 〈0. 05）. The level of TGF-131 was significantly higher in BTCC tissues than in normal tissues （76. 9% vs 26. 2%, P 〈 O. 05 ）. The expression level of E-cadherin in BTCC tissues was significantly low- ered in comparison with that in normal bladder epithelium （27.7% vs 89. 2% ,P 〈0. 05）. The expression rate of Vimentin was higher in BTCC tissues than in normal tissues （83.1% vs 20. 0% ,P 〈0. 05） ; （2） The expression of FOXQ1 serving as one new positive control factor of EMT was inversely correlated to E- cadherin, but positively to TGF-β1 and Vimentin. Conclusion FOXQ1 is related to the differentiation and metastasis of BTCC, and may induce EMT to promote BTCC metastasis.

关 键 词：上皮间质转化 膀胱移行细胞癌 核转录因子 叉头框蛋白Q1 

分 类 号：R737[医药卫生—肿瘤]