HPLC同时测定冬虫夏草中腺苷、虫草素和麦角甾醇  

Determination of Adenosine,Cordycepin and Ergosterol in Cordyceps sinensis by HPLC

作　　者：陈婷[1] 刘群[1] 莫灼康[1] 徐敏嫦[1] 陈润添[1] 

机构地区：[1]广东省东莞市人民医院,广东东莞523059

出　　处：《中国实验方剂学杂志》2013年第6期161-163,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:建立同时测定冬虫夏草药材中腺苷、虫草素和麦角甾醇的方法。方法:Diamosil C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水梯度洗脱,体积流量1.0 mL.min-1,柱温30℃,进样量10μL,检测波长260 nm。结果:腺苷、虫草素和麦角甾醇分别在0.032 2～0.805,0.015 4～0.385,0.084 3～2.107 5μg线性关系良好,回归方程分别为Y=2 573X+0.737 8;Y=2 631X+0.624 4;Y=2 315X+0.482 9,平均加样回收率分别为98.94%(RSD 1.16%),99.73%(RSD 2.19%),99.55%(RSD 2.02%)。结论:该方法准确性高,重复性好,可作为冬虫夏草药材的质量控制方法。Objective： To establish the quantitative analysis method for adenosine, cordycepin and ergosterol in Cordyceps sinensis by HPLC. Method： HPLC was performed on Diamosil C18 column（4.6 mm×250 mm, 5 μm）; the mobile phase consisted of methanol and water at a flow rate of 1.0 mL·min-1.The column temperature was set at 30 ℃, and the eluate was detected at 260 nm. Result： Quantitative analysis of HPLC showed that the linear ranges of adenosine, cordycepin and ergosterol were 0.032 2-0.805, 0.015 4-0.385 and 0.084 3-2.107 5 μg respectively.The average recoveries of adenosine, cordycepin and ergosterol were 98.94%（RSD 1.16%）, 99.73%（RSD 2.19%）, 99.55%（RSD 2.02%）respectively. Conclusion： The method set up is accurate, feasible and reliable, therefore available for the quality control of C. sinensis.

关 键 词：高效液相色谱法 冬虫夏草 腺苷 虫草素 麦角甾醇 

分 类 号：R284.1[医药卫生—中药学]