雷公藤内酯醇抑制CXCL11的表达对重症急性胰腺炎的影响  

The effect of triptolide by inhabit chemokine CXCL11 in severe acute pancreatitis

作　　者：吴兴[1] 张海峰[1] 强晖[1] 陈海琴[1] 周国雄[1] 

机构地区：[1]南通大学附属医院消化科,南通226001

出　　处：《南通大学学报（医学版）》2013年第1期31-34,共4页Journal of Nantong University(Medical sciences)

基　　金：南通市社会发展基金资助项目(S5054)

摘　　要：目的:探讨雷公藤内酯醇抑制CXCL11的表达对重症急性胰腺炎(severe acute pancreatitis,SAP)的治疗作用。方法:将72只SD大鼠随机分为3组:对照组,SAP组,雷公藤内酯醇治疗组,每组24只。牛黄胆酸钠建立SAP大鼠模型,治疗组予以雷公藤内酯醇治疗,分别检测各组不同时间点的血清淀粉酶,胰腺组织病理,免疫组化法、荧光定量检测胰腺CXCL11表达情况。结果:雷公藤内酯醇治疗组较SAP组血清淀粉酶值明显降低;胰腺病理与积分损伤减轻;胰腺免疫组化、荧光定量PCR提示雷公藤内酯醇治疗组较SAP组CXCL11表达明显减弱。结论:CXCL11参与了SAP发生发展,雷公藤内酯醇能显著抑制CXCL11的表达,减轻SAP时胰腺组织病理损伤。Objective： To investigate the effects of Triptolide by inhabit chemokine CXCLI 1 in severe acute pancreatitis（SAP）. Methods： Seventy-two SD rats were randomly divided into three groups： control group （C group）,SAP model group （P group）, Triptolide treatment group（T group）, and there were 24 rats in each group. SAP group was established by 4% sodium taurocholate. In T group,triptolide was injected into abdominal cavity immediately after the SAP model was sussessed. Serum amylase, the histolodic change of pancreas were measured at different time-point in each group. Protein in pancreas were decected by immunohistochemistry, and expression of CXCL11 mRNA in pancreas by fluorescence quantitative polymerase chain reaction. Results： Serum amylase and score of the histolodic change of pancreas were significantly lower in T group than P group at each time point, ImmunohistrochemistE~ and PCR showed expression of CXCL11 at each time point of T group were significantly lower compared with P group. Conclusions： CXCL11 may play an important role in the pathogenesis of SAP. Triptolide may ameliorate SAP and pancreatitis-associated complications by inhibiting the activity of CXCL11.

关 键 词：重症急性胰腺炎 CXCL11 雷公藤内酯醇 

分 类 号：R576[医药卫生—消化系统]