高肺血流性肺动脉高压大鼠肺动脉平滑肌中钙敏感受体表达和血浆中H_2S含量的检测及其意义  

Detection of calcium-sensing receptor expression in pulmonary arterial smooth muscle and H_2S content in plasma of rats with high pulmonary blood flow pulmonary hypertension and significance

作　　者：黄国雄[1] 何文武[1] 黄媛恒[2] 赵文[1] 宋绍辉[1] 郑晓宇[3] 王时俊[1] 石俊杰[1] 覃家锦[1] 

机构地区：[1]广西医科大学第一附属医院心胸外科,广西南宁530021 [2]广西医科大学实验生理科学实验中心,广西南宁530021 [3]广西壮族自治区南宁市第一人民医院心胸外科,广西南宁530021

出　　处：《吉林大学学报（医学版）》2013年第1期34-37,共4页Journal of Jilin University：Medicine Edition

基　　金：广西壮族自治区科学技术厅科技攻关与新产品试制项目资助课题(0816004-3)

摘　　要：目的:检测钙敏感受体(CaSR)在高肺血流性肺动脉高压(PH)大鼠肺动脉平滑肌中的表达水平及血浆中H2S含量,阐明两者在PH形成机制中的作用。方法:18只雄性SD大鼠随机分为对照组和PH组,对照组大鼠行单纯开胸,PH组大鼠行左肺切除手术。饲养35d后,应用右心导管法分别测定各组大鼠肺动脉平均压(PAMP),检测右心室/体质量(RV/BW)和右心室/左心室+室间隔[RV/(LV+S)]比值,光学显微镜观察肺血管结构的变化,分光光度法检测血浆H2S含量,应用实时荧光定量PCR方法检测大鼠肺动脉平滑肌细胞CaSR mRNA的表达水平。结果:PH组大鼠PAMP、RV/BW及RV/(LV+S)比值均明显高于对照组(P<0.05),光镜下肺小血管肌化程度明显增强,肺中、小肌型动脉相对中膜厚度明显增加(P<0.05)。PH组大鼠肺动脉平滑肌细胞CaSR mRNA的表达水平较对照组明显增高(P<0.05);PH组大鼠血浆中H2S含量较对照组明显降低(P<0.05)。大鼠肺动平滑肌细胞CaSR mRNA的表达水平与血浆H2S含量呈负相关(r=-0.931,P<0.05)。结论:高肺血流时肺动脉平滑肌细胞CaSR mRNA表达的改变可能通过改变内源性H2S的产生参与PH的形成。Objective To investigate the expression of calcium-sensing receptor （CaSR） in pulmonary arterial smooth muscle and hydrogen sulfide （HzS） in plasma of rats with high pulmonary blood flow pulmonary hypertension （PH）, and to clarify the role of both of them in the mechanism of PH. Methods 18 male SD rats were randomly divided into control group and PH group. The left lung resection surgery was performed in rats in PH group. 35 d after feeding, the right heart catheterization was used to measure mean pulmonary artery pressure （PAMP） of rats in control group and PH grout）; the right ventricular weight / body weight （RV / BW） and right ventricle / （left ventricle ＋ interventricular septum） [-RV/ （LV＋S）] ratios were detected, and the changes of pulmonary vascular structure were observed under optical microscope. Spectrophotometry was used to detect the plasma H2 S content. Real-time quantitative PCR was performed to detect the CaSR mRNA expression in pulmonary artery endothelial cells of rats in tow groups. Results The PAMP, the RV/BW and RV/ （LV＋ S） ratios in PH group were significantly higher than those in control group （P〈0.05）. Under light microscope, the pulmonary vascular muscle level was increased and the lung relative medial thickness of middle and small muscular arteries was also increased significantly in PH group （P〈0.05）. The CaSR mRNA expression in pulmonary artery endothelial cells in PH group was significantly higher than that in control group （P〈0.05） ; the plasma H2S content in PH group was significantly decreased compared with control group （P〈0.05）. Conclusion The changes of the CaSR mRNA expression in pulmonary artery endothelial cells in high pulmonary blood flow condition may be involved in PH by changing the generation of endogenous H2 S.

关 键 词：钙敏感受体 硫化氢 高肺血流 肺动脉高压 平滑肌 

分 类 号：R-332[医药卫生] R544.16