抑癌基因PTEN对舌鳞状细胞癌侵袭性及上皮-间质转化相关蛋白表达的影响  

Influence of PTEN in invasion and expression of epithelial-mesenchymal transition related protein in tongue squamous cell carcinoma

作　　者：郭婷婷[1] 张斌[1] 刘佳柏[2] 刘硕硕[1] 董肖婷[1] 

机构地区：[1]辽宁医学院附属第一医院口腔科,辽宁锦州121001 [2]吉林大学口腔医院牙体牙髓科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2013年第1期70-73,197,共4页Journal of Jilin University：Medicine Edition

基　　金：辽宁省教育厅高等学校科学研究项目资助课题(L2010315)

摘　　要：目的:探讨第10号染色体缺失的磷酸酶和张力蛋白同源物基因(PTEN)对舌鳞状细胞癌侵袭性的影响,阐明PTEN与舌鳞状细胞癌上皮间质转化(EMT)的关系。方法:舌鳞状细胞癌SCC-4细胞株分为未转染组(SCC-4细胞)、转染组(稳定转染了PTEN的SCC-4细胞)和空载体组(转染了空载体的SCC-4细胞)。用Transwell侵袭实验测定3组SCC-4细胞的侵袭能力;Western blotting法检测与EMT相关的E-cad、vimentin和snail蛋白的表达。结果:未转染组、空载体组和转染组SCC-4细胞在Transwell侵袭小室中培养36h后穿膜细胞数分别为82±5、80±4和42±5,其中未转染组与空载体组比较差异无统计学意义(P>0.05),转染组与未转染组比较差异有统计学意义(P<0.001)。与未转染组比较,转染组SCC-4细胞中E-cad、vimentin和snail蛋白表达差异有统计学意义(P<0.05),E-cad表达上调,vimentin和snail表达下调;空质粒组与未转染组比较差异无统计学意义(P>0.05)。结论:PTEN可能是通过抑制舌鳞状细胞癌SCC-4细胞株的EMT过程来降低其侵袭能力。Objective To investigate the influence of phosphatase and tensin homologue deleted on chromosome ten （PTEN） in invasion of tongue squamous cell carcinoma and to clarify the relationship between PTEN and epithelial- mesenchymal transition （EMT） in tongue squamous cell carcinoma. Methods The SCC-4 （non-transfection group）, pEGFP-PTEN-SCC-4 （transfection group）and pEGFP-SCC-4 （empty vector group） were cultivated at the same time. The cell invasion was observed by Transwell assay. The expressions of E-cad, snail and vimentin proteins were examined by Western blotting. Results The number of the cells which passed the membrane were.. 82±5, 80±4 and 42±5 in non-transfection group, empty vector group and transfection group 36 h after culture in Transwell assay there was no significant difference between non-transfection group and empty vector group （P〉 0.05）, but there was significant difference between non-transfection group and transfection group （P〈 0. 001）. Compared with non-transfection group, the E-cad expression was up-regulated （P〈0.05）, and the expressions of vimentin and snail were down-regulated （P〈0. 05） in transfection group; but there was no significant different between non-transfection group and empty vector group （P〉0.05）. Conclusion PTEN may reduce the invasion of tongne squamous cell carinoma SCC-4 cells by inhibiting the EMT process of tumor cells.

关 键 词：第10号染色体缺失的磷酸酶和张力蛋白同源物基因 舌鳞状细胞癌 上皮-间质转化 侵袭性 

分 类 号：R739.86[医药卫生—肿瘤]