牦犀胶颗粒的质量标准  

Quality Standard of Maoxijiao Granules

作　　者：姜建锋[1,2] 魏立新[1] 杜玉枝[1] 肖远灿[1] 朱洪梅[1,2] 杨红霞[1,2] 

机构地区：[1]中国科学院西北高原生物研究所,西宁市西关大街59号810001 [2]中国科学院研究生院,北京市100039

出　　处：《光谱实验室》2013年第2期623-628,共6页Chinese Journal of Spectroscopy Laboratory

基　　金：牦犀胶关键技术开发研究(2009GJG20044)

摘　　要：建立牦犀胶颗粒的质量标准。利用薄层色谱法及HPLC分别对牦犀胶颗粒中的黄芪、熟地进行定性鉴别。运用HPLC测定阿魏酸的含量。采用C_(18)柱,流动相为甲醇-0.1%冰乙酸(30:70,V/V),检测波长为321nm。结果表明,黄芪薄层色谱分离度较好,熟地HPLC梓醇色谱峰空白无干扰,阿魏酸的线性范围为0.15—1.5μg(r=0.9998,n=7),平均回收率为102.20%。所建立的定性、定量方法简单、准确、专属性强、重现性良好,可有效控制牦犀胶颗粒的质量。To establish the quality standard for Maoxijiao granules, TLC and HPLC were used respectively to identify Astragali Radix and Radix Rehmanniae Praeparata in them. HPLC was also used determine ferulic acid on a column of C18 with the mobile phase of methanol-0. 1M acetic acid （30 ： 70）, the detection wavelength was 321nm. The results showed that Astragali Radix could be identified by TLC with high specificity. The peak of catalpol chromatography of Radix Rehmanniae Praeparata was clear without the interference of the blank control. The linear range of ferulic acid was 0. 15-1.5μg（r=0. 9998,n= 7） ,and the average recovery was 100. 9%. The established qualitative and quantitative methods are simple, accurate and exclusive with good reproducibility, which can be used to control the quality of Maoxijiao granules effectively.

关 键 词：牦犀胶颗粒 质量标准 阿魏酸 梓醇 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]