β-环糊精存在下分光光度法测定微量锌  

Determination of Trace Zinc by Spectrophotometry in the Presence of β-Cyclodextrin

作　　者：那辉[1] 佟雷[2] 崔新宇[3] 

机构地区：[1]黑龙江幼儿师范高等专科学校学前系 [2]牡丹江医学院影像学院,黑龙江省牡丹江市150711 [3]牡丹江医学院公共卫生学院,黑龙江省牡丹江市150711

出　　处：《光谱实验室》2013年第2期635-637,共3页Chinese Journal of Spectroscopy Laboratory

摘　　要：基于β-环糊精(β-CD)存在下,Zn(Ⅱ)与2,4-二氯苯基荧光酮(DCIPF)的显色反应,建立了分光光度法测定微量锌的新方法。通过Zn(Ⅱ)-DCIPF-β-CD多元体系,来测量锌含量。体系最大吸收波长550nm,表观摩尔吸光系数ε=1.50×10~5L·mol^(-1)·cm^(-1),Zn(Ⅱ)含量在0.23—10.0μg/25mL范围内服从朗伯比尔定律,线性回归方程为A=0.211C(μg/25mL)+0.0122,r=0.9995,干扰离子较少。此法已用于自来水中锌含量的测定,结果令人满意。A new method for the determination of trace zinc by spectrophotometry was established, basing on the color reaction of Zn（ Ⅱ ） arid 2,4-dichlor-phenylfluorone in the presence of β-cyclodextrin（β-CD）. The zinc content was measured through the formed multi-component complex Zn （ Ⅱ ）-DCIPF-β-CD of high sensitivity. The maximum absorption wavelength of the complex was 550nm with apparent molar absorptivity of 1.50×10^5L·mol^-1·cm^-1.Zn（Ⅱ） content obeyed Lambert-Beer＇s law in the range of 0.23-10.0μg/25mL, the regression equation was A=0. 211C （μg/25mL） ＋0. 0122,r=0. 9995,with less interfering ions. The method has been applied to the determination of trace zinc in tap water with satisfactory results.

关 键 词：锌 分光光度法 2 4-二氯苯基荧光酮 表面活性剂 Β-环糊精 

分 类 号：O657.32[理学—分析化学]