乙型肝炎相关性肝癌患者血浆粒细胞集落刺激因子检测分析  

Analysis of serum granulocyte colony stimulating factor in patients with HBV related hepatocellular carcinoma

作　　者：乔桂芳[1] 孙焕琴[1] 李润青 刘宁[1] 刘金花[1] 王子康[1] 张永宏[1] 

机构地区：[1]首都医科大学附属北京佑安医院生物医学信息中心,100069 [2]北京清华医院检验科

出　　处：《北京医学》2013年第3期161-163,共3页Beijing Medical Journal

基　　金：北京市科技计划课题(D09050703560902);国家国际科技合作专项(2012DFA30850)

摘　　要：目的探讨粒细胞集落刺激因子(granulocyte colony stimulating factor,G-CSF)对乙型肝炎(乙肝)相关性肝癌的诊断价值。方法采用Luminex液相芯片方法检测87例乙肝相关性肝癌患者(肝癌组)、40例慢乙肝患者(慢乙肝组)及25例健康对照组(对照组)血浆中G-CSF水平,评价G-CSF对乙肝相关性肝癌的诊断价值。结果肝癌组患者血浆G-CSF的平均值为(1152.23±458.57)pg/ml,明显高于慢乙肝组[(137.69±89.52)pg/ml]和健康对照组[(111.04±49.42)pg/ml],差异均有统计学意义(P均=0.000)。3组间G-CSF检测结果的差异有统计学意义(F=157.760,P=0.000)。慢乙肝组和对照组G-CSF水平的差异无统计学意义(P=0.333)。肝癌分期不同组间G-CSF水平的差异有统计学意义(F=2.495,P=0.037)。血浆G-CSF诊断乙肝相关性肝癌的ROC曲线下面积(AUC)为0.998,以G-CSF≥256.00pg/ml为阳性诊断界值,G-CSF诊断乙肝相关性肝癌的敏感性为98.9%,特异性为100.0%。结论乙肝相关性肝癌患者G-CSF呈高水平表达,G-CSF可作为诊断乙肝相关性肝癌的候选指标。Objective To evaluate the diagnostic value of granulocyte colony stimulating factor （G-CSF） for HBV associated hepatocellular carcinoma. Methods Serum G-CSF were determined in 87 cases with hepatocellular carcinoma （HCC）, 40 cases with chronic hepatitis B and 25 normal control cases by Luminex suspension array. The diagnostic value of G-CSF was assessed for HCC. Results The serum G-CSF levels of 3 groups were significantly different （F = 157.760, P = 0.000）, and the mean level of G-CSF of HCC group was （l152.23＋458.57）pg/ml, which was significant higher than chronic hepatitis B group＇s and normal control group＇s[（137.69~89.52）pg/ml, （111.04~49.42）pg/ml ; all P = 0.000）]. No sig- nificant differences were observed in the G-CSF level between chronic hepatitis B group and normal control group. In ad- dition, on subgroup analysis, the serum level of G-CSF showed significant differences between HCC subgroups （ I a, I b, Ⅱ a, Ⅱ b, Ⅲ a, Ⅲ b）, P = 0.037. The G-CSF area under the ROC curve was 0.998, and at the cut-off value of 256.00 pg/ml, the G-CSF diagnostic sensitivity was 98.9% and diagnostic specificity was 100.0% for HCC. Conclusion In- creased levels of G-CSF are observed in patients with HCC, G-CSF is a good candidate tumor marker for HCC.

关 键 词：乙型肝炎 肝癌 粒细胞集落刺激因子 

分 类 号：R735.7[医药卫生—肿瘤]