用酶法测定血浆中1,6-二磷酸果糖含量  

Determination of Fructose-1,6-Diphosphatein Plasma by Enzymatic Method

作　　者：应汉杰[1] 顾海红 赵谷林[1] 欧阳平凯[1] 徐衡[2] 

机构地区：[1]南京化工大学制药与生命科学学院,江苏南京210009 [2]南京铁道医学院生物化学系,江苏南京210009

出　　处：《分析测试学报》2000年第4期43-45,共3页Journal of Instrumental Analysis

基　　金：国家"九五"重点科技攻关资助项目! (96 -C02 -03 -10)

摘　　要：建立了用酶法测定血浆中1,6_二磷酸果糖 (FDP)的方法 ,为含1,6_二磷酸果糖制剂的药代动力学研究提供基础。血浆用30 %(φ)的高氯酸沉淀蛋白后 ,分别在样品中加入0.56mg 还原型烟酰胺腺嘌啉二核苷酸(NADH),0.85u(酶活单位)3_磷酸甘油脱氢酶 (GDH)和60u磷酸甘油醛异构酶 (TIM)后15min在340nm处测定吸光值A1,然后加入0.25u醛缩酶 (ALD) ,15min后在340nm处测定吸光值A2。FDP在0～50mg/L范围内与ΔA(ΔA=A2-A1)呈线性关系 ,r=0.9997 ,FDP的平均回收率为99.7% (RSD为0.59% )。该法快速、灵敏、准确 ,样品处理简便易行。An enzymatic method for the determination of fructose _ 1,6 diphosphate(FDP) in plasma was established in order to study its pharmacokinetics. 0.56 mg nicotinamide adenine dinucleotide(NADH), 0.85 u glycerophosphate dehydrogenase(GDH) and 60 u triosephosphatisomerase(TIM) were added into sample after protein in plasma sample had been precipitated by 30% perchloric acid, and absorbance A1 was detected at a wavelength of 340 nm in 15 min. Then 0.25 u aldolase(ALD) was added into the sample and absorbance A2 was detected at 340 nm in 15 min. The mass concentration of FDP is linear with ΔA(A2-A1) in the range of 0～50 mg/L(r=0.999 7). The recovery of the method is 99.7%(RSD is 0.59%). The method is simple, rapid and precise.

关 键 词：二磷酸果糖 酶法 血浆 药代动力学 检测 

分 类 号：R977.9[医药卫生—药品] Q592.1[医药卫生—药学]