饲用羽毛肽粉的组分分离及分析  

Fractionation and Analysis of Feather Peptide for Feed

作　　者：姚清华[1,2] 黄薇[1,2] 林虬[1,2] 宋永康[1,2] 林香信[1,2] 胡兵 

机构地区：[1]福建省精密仪器农业测试重点实验室,福建福州350003 [2]福建省农业科学院中心实验室,福建福州350003 [3]福建天马饲料有限公司,福建福清350300

出　　处：《福建农业学报》2013年第1期22-26,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2010J01110);福建省农科院科技下乡"双百"行动项目(sbmb1239)

摘　　要：旨在研究饲用羽毛肽粉中肽分子量分布及各组分的氨基酸组成。利用AKTA purifier 100蛋白层析系统分离饲用羽毛肽粉溶液各组分。通过高分辨率Sephacryl S-100预装柱从样品中分离获得5种主要组分,利用氨基酸色谱分析各组分的氨基酸组成。通过回归方程计算出5种组分的平均分子量分别为:大于2 000 000、6 456.54、758.58、398.11、186.21。游离氨基酸组分5为天冬氨酸、苏氨酸、丝氨酸、谷氨酸;组分4为由天冬氨酸、苏氨酸、丝氨酸、甘氨酸、丙氨酸组成的二肽或三肽;组分3为天冬氨酸、苏氨酸、丝氨酸、谷氨酸、甘氨酸、丙氨酸组成的六肽或七肽;组分2的肽链长度与抗菌肽相近,氨基酸组成与组分1相同,较羽毛原毛少了胱氨酸、甲硫氨酸。结果提示:饲用羽毛肽粉富含小分子肽段,在饲料蛋白源领域具有潜在的应用价值。The aim of this study was fractionate and analysis of feather peptide for feed. 5 components contented in feather peptide meal were separated by AKTA purifier 100 with Sephacryl S-100. The molecular weight of them were as followed： 〉 2 000 000, 6 456.54, 758.58, 398.11, 186.21. Results of fractionation showed the fifth component were Asp, Thr, Ser, Glu. The fourth component were dipeptide or tripeptide, which content Asp, Thr, Ser, Gly, Ala. Amino acid composition of the third component, six peptide or seven peptide, were as followed： Asp, Thr, Ser, Glu, Gly, Ala. Peptide length of component 2, whose amino acid composition is the same as component 1, was close to antibacterial peptide. All these showed feather peptide meal was one kind of potential protein source for feed as it was rich in small peptides.

关 键 词：羽毛 饲料 肽 凝胶排阻 氨基酸 

分 类 号：S816[农业科学—饲料科学]