超临界流体萃取和高效液相色谱法测定银杏酸  

Determination of Ginkgolic Acid Using Supercritical Fluid Extrsction and High Performance Liquid Chromatography

作　　者：沈刚[1] 姚渭溪[1] 

机构地区：[1]中国科学院生态环境研究中心,北京100085

出　　处：《分析化学》2000年第8期985-988,共4页Chinese Journal of Analytical Chemistry

基　　金：中国科学技术部生命科学发展中心资助项目!(专题合同编号:94-Z-01-4)

摘　　要：介绍了一种用改性的超临界二氧化碳萃取和离线的反相高效液相色谱分析银杏叶提取物中银杏酸含量的方法。对萃取条件进行了优化，结果表明以硅胶为载体，在３０ＭＰａ、５５℃条件下，８％甲醇作改性剂时的超临界二氧化碳对银杏酸有最理想的回收率和选择性。最小检测限为０．４２ｍｇ／Ｌ。The determination method of ginkgolic acid in Ginkgo biloba extracts with modified supercritical carbon dioxide and reversed-phase high performance liquid chromatography was demonstrated. The conditions of extraction were optimized. The results showed that the recovery and the selectivity were the best under 30 MPa and 55℃ with 8% methanol modified supercritical carbon dioxide when the silica gel was used as matrix. The detection limit was 0.42 mg/L.

关 键 词：超临界二氧化碳萃取 银杏酸 测定 HPLC GBE 

分 类 号：O658[理学—分析化学]