阿霉素联合顺铂诱导宫颈癌细胞凋亡实验研究  

Experimental Study of ADM Combined with DDP on Human Cervical Cancer Cell Line CaSki in vitro

作　　者：赵文思[1] 吴薇[2] 任东明[1] 邹晓华[2] 刘朝奇[2] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学分子研究所,湖北宜昌443002

出　　处：《生物技术》2013年第1期67-70,共4页Biotechnology

基　　金：湖北省卫生厅青年科技人才基金项目(QJX2008-34);三峡大学教学研究项目(J2011013)资助

摘　　要：目的:研究化疗药物阿霉素(ADM)联合顺铂(DDP)对宫颈癌CaSki细胞株的增殖及凋亡的影响,并探讨其可能的相互作用机制。方法:应用MTT比色法检测不同浓度阿霉素、顺铂单独和联合应用对宫颈癌CaSki细胞的增殖抑制作用;同时RT-PCR法在mRNA水平上检测Bcl-2和TNF-α基因表达量的变化。结果:两种药物单独应用均可抑制CaSki细胞的增殖,联合用药(<12μg/mL)时具有协同抑制作用并与各药物单一应用比较具有显著性差异(P<0.05);阿霉素、顺铂作用CaSki细胞后能上调TNF-α基因和下调Bcl-2基因的表达。结论:宫颈癌CaSki细胞在化疗药物阿霉素和顺铂两药联合作用下通过诱导TNF-α和Bcl-2 mRNA表达量的变化发挥协同抑制作用,同时TNF-α的高表达增强了化疗药物阿霉素和顺铂诱导肿瘤细胞凋亡的敏感性,其机制主要与凋亡诱导效应有关。Objective：To study the effect of ADM combined with DDP on the growth inhibition of cervical cancer cell line CaSki. Method： Human cervical cancer cell line CaSki were cultured and then divided into 4 groups ： control group ; ADM group ; DDP group and combina- tion group （ ADM and DDP）. 48 h later the cell growth inhibition rate was detected by MTY assay. RT - PCR was used to detect the mRNA expression of TNF - ct and Bcl -2. Result：The cervical cancer CaSki cell proliferation was inhibited by ADM combined with DDP in dose dependently manner. ADM combined with DDP significantly enhanced the mRNA expression of TNF - a and Bcl - 2. Conclusion ： The growth inhibitory effect of ADM combined with DDP is enhanced through induction of TNF - ct on human cervical cancer cells to the mech- anism of induction of apoptosis.

关 键 词：宫颈癌 阿霉素 顺铂 TNF-Α BCL-2 凋亡 

分 类 号：R737.9[医药卫生—肿瘤]