肺病理取材定量比较研究及其诊断学意义  

Quantitative comparison of lung pathological processing and its diagnostics significance

作　　者：李海胜[1] 杜鹃[1] 王海燕[1] 岳彩黎[1] 杨策[1] 蒋建新[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所创伤、烧伤与复合伤国家重点实验室,重庆400042

出　　处：《解放军医学杂志》2013年第3期180-184,共5页Medical Journal of Chinese People's Liberation Army

基　　金：国家重点基础研究发展计划(973计划)(2012CB518104)~~

摘　　要：目的通过定性和定量的方法比较单纯气管内固定、单纯肺循环固定和气管肺循环联合固定对肺泡结构和细胞抗原的保存效果。方法 15只健康SD大鼠随机均分为单纯气管内固定组、单纯肺循环固定组和气管肺循环联合固定组。分别按3种方法固定,石蜡包埋,行HE和免疫荧光染色,采用定性观察和体视学定量分析不同方法的固定效果。结果定性观察发现,单纯肺循环固定和气管肺循环联合固定对胞质和胞核抗原的保存效果优于单纯气管内固定。定量分析发现,气管肺循环联合固定组的肺泡线性截距大于单纯气管内固定组和单纯肺循环固定组(P<0.05);气管肺循环联合固定组的肺实质体积密度和肺间隔厚度最小,单纯气管内固定组次之,单纯肺循环固定组最大(P<0.05)。结论气管肺循环联合固定可以更好地保存肺泡结构和细胞抗原,适合基于形态定量分析的病理基础和临床诊断研究。Objective To qualitatively and quantitatively compare the effect of tracheal inflation method, vascular perfusion method and combined trachea-vascular perfusion method on alveolar structure and cell antigen preservation. Methods Fifteen healthy Sprague Dawley rats were randomly divided into three groups （5 each）： tracheal inflation group, vascular perfusion group and combined trachea-vascular perfusion group. After lungs were respectively fixed with the methods mentioned above, tissue blocks were paraffin embedded and sectioned. HE staining and immunofluorescence staining were then performed for qualitative observation and stereological analysis of the fixation effect. Results Qualitative study showed that the fixation effect of cytoplasm and nuclear antigen was better in vascular perfusion group and combined trachea-vascular perfusion group than in tracheal inflation group. Stereological analysis showed that the alveolar linear intercept in combined trachea-vascular perfusion group was longer than that in tracheal inflation and vascular perfusion group （P〈0.05）. The lung parenchyma volume density and alveolar septum thickness of combined trachea-vascular perfusion group were smallest among all the three groups, followed by tracheal inflation group and vascular perfusion group in turn （P〈0.05）. Conclusions The combined trachea-vascular perfusion method could not only preserve better alveolar structure but also display perfect cellular antigen. Therefore, it is suitable for the morphometry-based researches on lung pathological and clinical diagnosis.

关 键 词：病理学 肺 组织固定 肺体视学 

分 类 号：R36-33[医药卫生—病理学]