顺磁性铁纳米粒促进人淋巴细胞对乳腺癌细胞的体外杀伤  

Iron oxide nanoparticle promotes the anti-tumor cytotoxicity against breast cancer cells by human lymphocyte in vitro

作　　者：张亮[1] 孙昭[2] 段金虹[1] 熊非[3] 张宇[3] 顾宁[3] 许海燕[1] 杨先达[1] 

机构地区：[1]中国医学科学院基础医学研究所,北京100005 [2]北京协和医院肿瘤内科,北京100730 [3]东南大学生物科学与医学工程学院,南京210009

出　　处：《基础医学与临床》2013年第4期387-390,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(81071870);国家重大科学计划973项目(2011CB933504)

摘　　要：目的探索顺磁性氧化铁纳米颗粒是否能调节由肿瘤裂解蛋白所诱发的针对人乳腺癌细胞(MCF-7)的抗肿瘤免疫反应。方法将纳米铁颗粒与肿瘤裂解蛋白进行共价连接形成纳米铁-蛋白复合物,用裂解蛋白、纳米铁、纳米铁-蛋白复合物分别刺激人外周血单个核细胞(PBMC),再将刺激后的PBMC与MCF-7细胞相混合,用MTS方法检测对肿瘤细胞的杀伤率。结果纳米铁能与60%的肿瘤裂解蛋白相连接,并形成较大的纳米颗粒。与单纯的肿瘤蛋白相比,纳米铁-肿瘤蛋白的复合物能够显著提升PBMC对MCF-7细胞的杀伤,而纳米铁本身对MCF-7无直接杀伤作用。结论纳米铁-肿瘤蛋白的复合物能够显著增强PBMC在体外杀伤人乳腺癌细胞的能力。Objective To explore whether superparamagnetic iron oxide nanoparticle （SPIO） will influence the an- ti-tumor immunity triggered by tumor lysate protein against human MCF-7 breast cancer cells （MCF-7） in vitro. Methods SPIO was covalently conjugated with tumor lysate protein to form SPIO-protein complex. Human periph- eral blood mononuclear cells （ PBMC ） were stimulated by tumor lysate protein, SPIO, or SPIO-protein complex separately. The treated PBMC were mixed with MCF-7 cells and tumor inhibition was evaluated by MTS assay. Re- sults The SPIO conjugated with 60% of tumor lysate protein and f6rmed a larger nano-structure. Compared with tumor lysate protein, SPIO-protein complex significantly enhanced the anti-tumor response by PBMC, whereas SPIO alone produced no cytotoxicity against MCF-7 cells. Conclusions The SPIO-protein complex is capable of enhan- cing the anti-cancer immune reaction against MCF-7 cancer cells by PBMC in vitro.

关 键 词：顺磁性氧化铁纳米颗粒 肿瘤裂解蛋白 抗肿瘤免疫疗法 MCF-7细胞 

分 类 号：R737.9[医药卫生—肿瘤]