CYP1A1和NQO1及EPHX1基因多态性与膀胱癌易感相关性分析  

Relationship between genetic polymorphisms of CYP1A1,NQO1 and EPHX1 and susceptibility to bladder cancer

作　　者：傅晶[1] 陈必成[2] 

机构地区：[1]绍兴第二医院泌尿外科,浙江绍兴312000 [2]温州医学院附属第一医院外科实验室,浙江温州325000

出　　处：《中华肿瘤防治杂志》2013年第1期10-14,共5页Chinese Journal of Cancer Prevention and Treatment

摘　　要：目的:探讨细胞色素P450 1A1(CYP1A1)、依赖还原型辅酶Ⅰ/Ⅱ醌氧化还原酶1(NQO1)和环氧化物水解酶(EPHX1)基因多态性与浙江地区膀胱癌易感性的关系。方法:采用病例对照研究方法,应用相对的2对引物-聚合酶链反应技术(PCR-CTPP),对99例膀胱癌患者和100例非肿瘤患者的CYP1A1A4889G、NQO1C609T和EPHX1A416G基因型进行检测,并分析其与膀胱癌易感性的关系。结果:NQO1C609T基因型分布频率在膀胱癌组和对照组之间的差异有统计学意义,P<0.05;携带609TT基因型的个体罹患膀胱癌的风险是携带609CC基因型个体的2.448倍(95%CI为1.125～5.326)。CYP1A1A4889G和EPHX1A416G基因型分布频率在两组间的差异均无统计学意义,P>0.05。携带NQO1 609TT与EPHX1 416AA两高危基因型的个体罹患膀胱癌的风险是同时携带两低危基因型个体的4.181倍(95%CI为1.465～11.934)。相类似的,携带CYP1A1 4889AG/GG、NQO1 609TT与EPHX1 416AA三高危基因型的个体罹患膀胱癌的风险则为4.765倍(95%CI为0.895～25.364)。结论:NQO1C609T基因多态性可能与膀胱癌的易感性相关,携带NQO1 609TT基因型的人群易患膀胱癌。而且,基因与基因(NQO1 609TT与EPHX1 416AA,CYP1A1 4889AG/GG、NQO1 609TT与EPHX1 416AA)的交互作用增加了这种风险。OBJECTIVE： To explore the relationship between genetic polymorphisms of CYP1A1, NQO1 and EPHX1 and susceptibility to bladder cancer. METHODS： Polymerase chain reaction with confronting two-pair primers （PCR-CTPP） was employed to assess the genotype frequencies of CYPIA1 A4889G, NQO1 C609T and EPHX1 A416G both in 99 patients with bladder cancer and 100 cancer-free controls. RESULTS：The difference of NQO1 C609T genotype frequencies between cases and controls had statistical significance（P〈0. 05）, and bladder cancer risk in patients with 609TT genotype was 2.448-fold as high as that in patients with 609 CC genotype （ 95%CI = 1.125 -5.326 ）. For the genotype frequencies of CYP1A1 A4889G and EPHX1 A416G, there were all no statistical significance between cases and controls （P〉0. 05）. Combination of the two high-risk genotypes NQO1 609TT and EPHX1 416AA revealed that bladder cancer risk was increased up to 4. 181 times （95%CI= 1. 465-11. 934） compared with the combination of the two low risk genotypes of these loci. Similarly, the risk was 4. 765 times （95%CI= O. 895-25. 364）for the combined three highrisk genotypes CYP1A1 4889AG or GG, NQO1 609TT and EPHX1 416AA. CONCLUSIONS.. These findings demonstrat that NQO1 C609T genetic polymorphism should likely be associated with susceptibility to bladder cancer. People with NQO1 609TT genotype have higher bladder cancer risk. Furthermore, the interactions of gene-gene （NQO1 609TT-EPHX1 416AA,CYP1A1 4889AG or GG-NQO1 609TT-EPHX1 416AA） increase this risk substantially.

关 键 词：膀胱肿瘤 细胞色素P450 1A1 依赖还原型辅酶I Ⅱ醌氧化还原酶1 环氧化物水解酶 多态现象 遗传 

分 类 号：R737.14[医药卫生—肿瘤]