^(125)Ⅰ粒子植入治疗人中分化胃癌裸鼠移植瘤  

Implantation of radioactive seed ^(125)Ⅰ for the treatment of implanted tumor of moderately differentiated human gastric cancer cell line in nude mice

作　　者：邹雷[1,2,3] 罗开元[1] 马振桓[1,2] 李波[1] 李晓刚[1,2] 许建彪[1,2] 

机构地区：[1]昆明医科大学第四附属医院普外科,昆明市650021 [2]昆明医科大学第四附属医院 [3]云南省第一人民医院肝胆外科

出　　处：《中国肿瘤临床》2013年第5期248-252,共5页Chinese Journal of Clinical Oncology

基　　金：云南省科技厅应用基础研究重点项目(编号:2008CC002)资助~~

摘　　要：目的:探讨不同剂量125Ⅰ粒子组织间植入对人中分化胃癌细胞AGS裸鼠移植瘤生长的抑制效果及机制。方法:构建20只人中分化胃癌AGS的裸鼠移植瘤模型,随机分为4组,每组5只。3个治疗组分别接受14.8MBq(A组)、22.3MBq(B组)、29.6MBq(C组)的125I粒子治疗,对照组(D组)不接受治疗。绘制肿瘤生长曲线,计算抑瘤率。通过检测细胞增殖核抗原(PCNA),了解不同剂量125I粒子对肿瘤细胞增殖作用的影响。原位末端标记法(TUNEL)检测不同剂量125I粒子诱导肿瘤细胞凋亡的情况。结果:中、高剂量组肿瘤体积、重量降低明显;不同剂量组的抑瘤率分别为(A组10.65%、B组28.65%、C组37.26%),中、高剂量治疗组PCNA的表达降低明显,中、高剂量治疗组凋亡发生明显,以上各组中、高剂量组与对照组相比均有显著统计学差异(P<0.05)。结论:高、中剂量125I粒子治疗中分化胃癌效果较好;诱导细胞凋亡、PCNA表达下调,可能是抑制肿瘤生长的部分原因。Objective： This study aimed to describe the inhibitory effects of different doses of radioactive seed ^125Ⅰ on the treatment of implanted tumor of moderately differentiated human gastric cancer cell line in nude mice. Methods： We developed 20 nude mice models with implanted tumor of moderately differentiated human gastric cancer cell line. The mice were randomly divided into four groups with five mice in each group. Three groups were treated with radioactive seed ^125Ⅰ at different doses： 14.8 MBq （group A）; 22.3 MBq （group B）; and 29.6 MBq （group C）. The control group （group D） was not subjected to radioactive seed ^125Ⅰ implantation. We constructed the growth curves of the implanted tumor and calculated the tumor inhibition rate in each group. The effects of the radioactive seed ^125Ⅰ implanted at different doses on tumor propagation were estimated by detecting the proliferating cell nuclear antigen （PCNA）. The effects of induced cell apoptosis were determined by TdT-mediated dUTP nick-end labeling. Results： The volume and the weight of the implanted tumor in groups C and D were significantly reduced （P〈0.05）. The tumor inhibition rates of groups A, B, and C were 10.65%, 28.65%, and 37.26%, re- spectively. The PCNA expressions in groups B and C were also significantly reduced （P〈0.05）. The inductive effects of apoptosis in groups B and C were significantly greater than those in the control group （P〈0.05）. Conclusion： Medium- and high-dose radioactive seed ^125Ⅰcould induce apoptosis and reduce PCNA expression. These findings may partially account for the inhibition of tumor growth. Thus, this method could be effectively used to treat human gastric cancer.

关 键 词：125Ⅰ粒子 近距离放疗 胃癌 植入 裸鼠 

分 类 号：R735.2[医药卫生—肿瘤]