Gli1和PDGF-D在结直肠癌中的表达及其临床意义  

Expression of Gli1 and PDGF-D in colorectal cancer and its clinical significance

作　　者：寇耀[1] 魏正强[1] 杨勇[1] 张刘平[1] 陈志雄[1] 汤为学[2] 

机构地区：[1]重庆医科大学附属第一医院胃肠外科,重庆市400016 [2]重庆市神经病学重点实验室

出　　处：《中国肿瘤临床》2013年第5期261-265,共5页Chinese Journal of Clinical Oncology

摘　　要：目的:探讨Gli1和PDGF-D在结直肠癌中的表达及其临床意义。方法:采用免疫组织化学S-P法、Western blot法分别定性、定量检测54例结直肠癌组织及其对应30例癌旁正常组织中Gli1和PDGF-D蛋白表达情况并结合临床病例资料进行分析。结果:免疫组织化学结果显示Gli1和PDGF-D在结直肠癌中的阳性表达率分别为(72.22%、74.07%),与正常组织的阳性表达率(6.67%、33.33%)相比显著增高,差异具有统计学意义(P<0.05)。Western blot检测Gli1和PDGF-D在癌组织中相对表达量分别为0.731±0.238和0.817±0.288,与正常组织0.196±0.041和0.236±0.060相比,两者表达也显著增高,差异具有统计学意义(P<0.05);Gli1和PDGF-D的表达与肿瘤的分化程度、淋巴结转移有关(P<0.05),PDGF-D还与肿瘤分期有关(P<0.05);两者表达与患者的年龄、性别、肿瘤大小、侵袭深度均无明显相关性(P>0.05);Gli1和PDGF-D在结直肠癌中的表达呈正相关(P<0.05)。结论:Gli1和PDGF-D在结直肠癌中异常高表达,可能共同参与并促进结直肠癌的恶性进程。Objective： This work aimed to investigate the expression of the Glil and PDGF-D proteins in colorectal cancer and its clinical significance. Methods： Immunohistochemistry and Western blot analysis were used to detect the expression of Gli 1 and PDGF-D in tissue samples from 54 colorectal cancer case patients and adjacent normal tissues from 30. The results were obtained after clinico- pathological features were analyzed. Results： Immunohistochemical assay showed that the positive rates of Gli 1 and PDGF-D in the colorectal cancer tissue samples （72.22% and 74.07%, respectively） were significantly higher than those in the normal tissues （6.67% and 33.33%, respectively） （P〈0.05）. Western blot analysis revealed that the relative expression levels of Glil and PDGF-D in the colorectal cancer tissue samples （0.731±0.238 and 0.817±0.288, respectively） were also significantly higher than those in the normal tissue samples （0.196±0.041 and 0.236±0.060, respectively） （P〈0.05）. The expression of Glil and PDGF-D was positively correlated with the differentiation of colorectal cancer and lymph node metastasis, and the expression of PDGF-D was also positively correlated with the World Health Organization tu- mor-node-metastasis staging system （P〈0.05）. Neither gene was correlated with patient sex, patient age, tumor size, and depth of tumor invasion （P〉0.05）. The expression of Glil and that of PDGF-D were positively correlated （P〈0.05）. Conclusion： Glil and PDGF-D are expressed in colorectal cancer at abnormally high levels. They may act in concert and play an important role in the carcinogenesis of the disease.

关 键 词：GLI1 PDGF-D 结直肠癌 免疫组织化学 WESTERN BLOT 

分 类 号：R735.3[医药卫生—肿瘤]