TSH与脂肪细胞NF-κB、肿瘤坏死因子-α蛋白水平相关性的研究  

Correlation of thyroid-stimulating hormone with NF-kB p65 and TNF-αin adipocytes

作　　者：张雅静[1,2] 赵伟[1,2] 张宏[1,2] 吴晓明[1,2] 刘佳[1,2] 张琳[1,2] 李维[1,2] 

机构地区：[1]天津医科大学代谢病医院内分泌研究所,300070 [2]卫生部激素与发育重点实验室,300070

出　　处：《中华内分泌代谢杂志》2013年第3期257-259,共3页Chinese Journal of Endocrinology and Metabolism

基　　金：天津市应川基础及前沿技术研究计划重点项目（10JCZDJE19900）

摘　　要：ELISA法榆洲大鼠血清TSH水平，Western印迹法检测大鼠脂肪组织及3T3-L1脂肪细胞NF1-kBp65、肿瘤坏死川子（TNF）-α蛋白水平。结果显示TSH与NF-kKBp65、TNF-α蛋白表达正相关，与胰岛素作用指数负相关．TSH促进情眈细胞NF-KBp65蛋白表达及TNF-α分泌呈剂量依赖性（P〈0．05）。Serum TSH level was determined by Enzyme-l,inked hnmunosorbent Assay （ELISA）, The expression of NF-KB p65 and tumor necrosis factor-α（TNF-α） were quantified by Western blotting. The results showed that serum TSH concentration was positively correlated with the expression of NF-s：B p65 and TNF-α, and negatively correlated with insulin activity index. The levels of TNF-αand NF-xB p65 both were increased by TSH in a dose dependent manner（ P〈0.05 ）.

关 键 词：胰岛素抵抗 脂肪细胞 促甲状腺激素 肿瘤坏死因子-Α 核转录因子-ΚB 

分 类 号：R363[医药卫生—病理学]