肝细胞性肝癌相关miRNA的筛选和鉴定  

Screening and identification of hepatocellular carcinoma-related miRNA

作　　者：沈雁兵[1] 马德宾[1] 邹丽云[2] 谢谆怡[2] 万瑛[2] 陈平[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所肝胆外科,重庆400042 [2]第三军医大学大坪医院基础部免疫学研究所,重庆400042

出　　处：《免疫学杂志》2013年第4期310-313,共4页Immunological Journal

基　　金：第三军医大学青年人才创新基金(2010XQN09);重庆市自然科学基金(2010BB5173);国家自然科学基金(81270523)

摘　　要：目的检测正常肝细胞与肝癌细胞中微小RNA(microRNA,miRNA)的表达差异,探讨其参与肝癌发病的可能机制。方法首先利用miRNA微阵列试剂盒,以正常肝细胞株L02为对照,筛选出肝癌细胞株HepG2中差异表达的miRNA,然后采用定量RT-PCR以及另外1株肝癌细胞株Huh7对筛选结果进行验证,最后选取肝癌细胞特异性的miRNA,运用生物信息学技术对其靶基因进行预测,并对其靶基因功能进行分类。结果发现9种表达下调的miRNA,分别为let-7a、miR-10a、miR-107、miR-124a、miR-132、miR-133a、miR-154、miR-302c、miR-373。靶基因预测结果显示其主要参与了19种生物学事件,且大部分生物学事件与肿瘤的发病机制有关。结论发现肝癌细胞中9种表达下调的miRNA,且其潜在靶基因参与的生物学事件中的大部分与肿瘤的发病机制有关。This study designed to explore miRNA expression profiles between hepatocellular carcinoma （HCC） cell lines and normal liver cell lines and investigate its role involved in carcinogenesis. Here we use a microRNA qPCR array method to analysis the expression level of 96 human miRNAs in HCC cell line HepG2 and normal liver cell line L02; then the screening results were validated by another HCC cell line Huh7 through Reahime PCR. Finally an in silico strategy was employed to obtain the predicted target genes of the miRNA. Total of 9 microRNAs were identified as down-regulated in HCC versus normal liver, including let-7a, miR-10a, miR-107, miR-124a, miR-132, miR-133a, miR-154, miR-302c, miR-373. Target genes of the 9 microRNAs have been found to involve in 19 biological pathways and most of the pathways have been implicated in carcinogenesis.

关 键 词：肝细胞性肝癌 MICRORNA 表达谱 

分 类 号：R735.7[医药卫生—肿瘤]