肿瘤坏死因子相关凋亡诱导配体对肿瘤细胞PRDXs表达影响的观察  

Effect of tumor necrosis factor-related apoptosis-inducing ligand on PRDXs expression

作　　者：张海燕[1] 孟欣[2] 都镇先[3] 邓娓娓[1] 王华芹[2] 

机构地区：[1]中国医科大学附属第一医院老年病干诊科,辽宁沈阳110001 [2]中国医科大学基础医学院生化与分子生物教研室,辽宁沈阳110001 [3]中国医科大学附属第一医院内分泌科,辽宁沈阳110001

出　　处：《中华肿瘤防治杂志》2013年第6期427-430,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：辽宁省科技攻关项目(2010225032);辽宁省教育厅资助项目(2009A765)

摘　　要：目的:探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)对肿瘤细胞中过氧化氧化还原蛋白(PRDXs)表达的影响及其机制。方法:选取肿瘤细胞,设立对照组和TRAIL处理组;用实时定量PCR法和蛋白质印迹法检测PRDXs在各种肿瘤细胞中的表达;用PCR法克隆PRDX4启动子,用萤光素酶含量测定其含量。结果:与对照组相比,TRAIL处理组中PRDX1、PRDX2、PRDX3、PRDX5和PRDX6mRNA及蛋白表达差异无统计学意义,P>0.05;PRDX4mRNA和蛋白的表达显著降低,P<0.01;放线菌素D处理8h时,TRAIL处理组与对照组中PRDX4mRNA降解近50%,差异无统计学意义,P>0.05;TRAIL显著降低pPRDX4/-979的活性呈剂量依赖方式。结论:TRAIL在转录水平上下调肿瘤细胞中PRDX4基因的表达,对PRDX4mRNA的稳定性没有影响。OBJECTIVE： To investigate the mechanism of Peroxiredoxins （PRDXs） expression in the panel of cancer cells affected by tumor necrosis factor-related apoptosis-inducing ligand（TRAIL）. METHODS： A panel of cancer cells were selected and set up control group and TRAIL treatment groups for investigation; the expression of PRDXs mRNA and protein in the panel of cancer ceils was confirmed by real-time RT-PCR and Western blot, respectively; Generation of PRDX4 promoter subcloned by PCR. The luciferase activity was determined using the dual-luciferase reporter assay sys- tem. RESULTS.. TRAIL significantly reduced PRDX4 mRNA and protein levels （P〈 0. 01）, while PRDX1, PRDX2, PRDX3,PRDX5 and PRDX6 had no obvious effects （P〉0.05） when compared with control group; PRDX4 mRNA in HeLa treated with control or TRAIL degraded approximately by 50% after 8 h treatment with actinomycin D,there was no significant difference between two groups（P〉0.05）. TRAIL significantly decreased pPRDX4/_979 activity in a dose- dependent manner. CONCLUSION： TRAIL suppressed PRDX4 expression at the transcriptional level, have no effect on PRDX4 mRNA stability.

关 键 词：肿瘤坏死因子相关凋亡诱导配体 过氧化氧化还原蛋白4 肿瘤 凋亡 

分 类 号：R730.2[医药卫生—肿瘤]