依达拉奉后处理联合远隔缺血后处理对大鼠心肌缺血再灌注损伤的影响  

Effects of edaravone postconditioning and remote ischemic postconditioning on myocardial ischemia/reperfusion injury in rats

作　　者：李恒昌[1] 许立新[1] 李知平[2] 张曙[2] 索琨[1] 佘守章[1] 付晓东[3] 

机构地区：[1]广州市第一人民医院麻醉科,510180 [2]广东省东莞市人民医院麻醉科 [3]中山大学生理学教研室

出　　处：《中华麻醉学杂志》2013年第1期99-102,共4页Chinese Journal of Anesthesiology

基　　金：广州市医药卫生科技项目（201102A213081）;广东省医学科研基金项目（A2012786）

摘　　要：目的评价依达拉奉后处理联合远隔缺血后处理对大鼠心肌缺血再灌注损伤的影响。方法健康雄性SD大鼠40只，8周龄，体重250～300g，采用随机数字表法，将其分为5组（n=8）：假手术组（S组）、缺血再灌注组（I／R组）、依达拉奉后处理组（E组）、远隔缺血后处理组（P组）、依达拉奉联合远隔缺血后处理组（EP组）。采用结扎左冠状动脉前降支30min，再灌注180min制备心肌缺血再灌注模型。E组和EP组再灌注前1rain静脉注射依达拉奉3mg／kg；P组和EP组左冠状动脉结扎20min时实施远隔后处理：用止血带结扎大鼠双后肢，持续10min。于心肌缺血再灌注期间记录左心室峰压（LVSP）、左心室舒张末压（LVEDP）、左室内压最大上升速率（＋dp／dtmax）、左室内压最大下降速率（-dp／dtmax）。结果与S组比较，其它各组再灌注期间LVSP、＋dp／dtmax、-dp／dtmax降低，LVEDP升高（P〈0．05）；与I／R组比较，E组、P组及EP组再灌注期间LVSP、＋dp／dtmax、-dp／dtmax升高，LVEDP降低（P〈0．05）；与E组和P组比较，EP组再灌注期间LVSP、＋dp／dtmax、-dp／dtmax升高，LVEDP降低（P〈0．05）。结论依达拉奉后处理联合远隔缺血后处理可减轻心肌缺血再灌注损伤，且效果强于两者单独应用。Objective To evaluate the effects of edaravone postconditioning and remote ischemic postconditioning on myocardial ischemia/reperfusion （I/R） injury in rats. Methods Forty male Sprague-Dawley rats, aged 8 weeks, weighing 250-300 g, were randomly divided into 5 groups （ n = 8 each） ： sham operation group （group S）; group I/R; edaravone postconditioning group （group E）; remote ischemic postconditioning group （group P）; edaravone postconditioning and remote isehemic postconditioning group （group EP）. Myocardial I/R was induced by occlusion of anterior desending branch of left coronary artery for 30 rain followed by 180 rain reper- fusion. Edaravone 3 mg/kg was injected intravenously at 1 min before reperfusion in groups E and EP. The animals underwent 10 min ischemia of bilateral hind limbs starting from 20 rain of myocardial ischemia in groups P and EP. Left ventricular systolic pressure （LVSP）, left ventricular end-diastolic pressure （LVEDP） and ± dp/dtmax, were measured and recorded during reperfusion. Results Compared with group S, LVSP and ± dp/dtmax were significantly decreased and LVEDP was increased in the other groups （P 〈 0.05）. LVSP and ± dp/dtmax were signifi- cantly higher and LVEDP was lower during reperfusion in groups E, P and EP than in group I/R, and in group EP than in groups E and P （P 〈 0.05）. Conclusion Edaravone postconditioning and remote ischemic postconditioning can alleviate myocardial I/R injury and offers better efficacy than either alone.

关 键 词：自由基清除剂 心肌再灌注损伤 缺血后处理 

分 类 号：R743.3[医药卫生—神经病学与精神病学]