光裸星虫提取物对血栓形成的影响  

Effects of extracts of Sipunculus nudus Linnaeus on thrombosis

作　　者：刘欣[1] 唐于平[1] 刘睿[1] 郭建明[1] 丁少雄[2] 林祥志[3] 林汝榕[3] 段金廒[1] 

机构地区：[1]南京中医药大学江苏省方剂高技术研究重点实验室,江苏南京210046 [2]厦门大学海洋与地球学院,福建厦门361005 [3]国家海洋局第三海洋研究所,福建厦门361005

出　　处：《中国海洋药物》2013年第2期17-25,共9页Chinese Journal of Marine Drugs

基　　金：海洋公益性行业科研专项(201205024)资助

摘　　要：目的通过对家兔体外血浆血小板聚集和凝血功能的影响、对大鼠动-静脉血流旁路血栓形成的影响、对DPPH自由基的清除作用及对纤维蛋白的溶解作用,综合评价星虫提取物对血栓形成的影响。方法以二磷酸腺苷(ADP)诱导血小板聚集,采用比浊法观察星虫提取物对家兔体外血浆血小板6min聚集率和最大聚集率的影响;采用凝固法来评价星虫提取物对家兔体外血浆凝血酶原时间(PT)、活化部分凝血激酶时间(APTT)及凝血酶时间(TT)的影响;采用动静脉旁路模型,来研究星虫提取物对大鼠动静脉旁路血栓形成的影响;采用2,2-二苯基-1-苦肼基(DPPH)自由基清除率法,来评价星虫提取物清除OH自由基的作用;通过纤维蛋白平板实验研究星虫提取物的纤溶活性。结果星虫提取物能明显抑制ADP诱导的家兔体外血小板6min聚集率和最大聚集率,能明显延长家兔PT、APTT和TT,明显抑制大鼠动静脉旁路血栓形成,减轻血栓湿重和干重;具有清除OH自由基的作用以及不同程度地溶解纤维蛋白的作用。结论星虫提取物具有很好的抗血栓形成的作用。Objective To evaluate the thrombotic activity of Sipunculus nudus extracts by investigating their effects on rabbit plasma platelet aggregation and coagulation function in vitro, and the effect on the model of artery-vein bypass thrombosis in rats, as well as capacity of eliminating DPPH free radi- cals and dissolving fibrin. Methods Inhibition rates of platelet aggregation in vitro were determined in the model of platelet aggregation induced by adenosine diphosphate. Their anticoagulative effects were also evaluated with prothrombin time （PT）, activated partial thromboplastin time （APTT） and throm- bin time （TT）. The model of artery-vein bypass thrombosis was established to observe their antithrom- botic effect. The antioxidant effects were investigated by DPPH free radical scavenging capability test. The fibrinolytic activity was determined by fibrin plate. Results The results showed that the extracts had significant inhibition effect on platelet aggregation, and could obviously prolong PT, APTT and TT, inhibited the artery-vein bypass, reduced the thrombus weight significantly （wet or dry）. And the extracts of S. nudus also could eliminate DPPH free radicals and dissolve fibrin at different degree. Con- clusion Extracts of S. nudus had strong antithrombotic effect.

关 键 词：星虫 血栓形成 抗血栓 血小板聚集 凝血酶 抗氧化 纤维蛋白平板 

分 类 号：R965[医药卫生—药理学]