miRNA-21对其靶基因PTEN在前列腺癌中表达调控机制的研究  

The study of expression and regulatory role of microRNA-21(miR-21)on PTEN in prostate cancer

作　　者：肖黎[1] 金艳阳[2] 佟明[2] 

机构地区：[1]辽宁医学院,辽宁锦州121001 [2]辽宁医学院附属第一医院泌尿外科

出　　处：《现代预防医学》2013年第6期1112-1115,共4页Modern Preventive Medicine

摘　　要：目的观察前列腺癌组织和细胞中miRNA-21和PTEN的表达情况,探讨miRNA-21对PTEN的调控。方法应用实时荧光定量PCR和Westernblot检测正常前列腺组织、前列腺癌组织、非依赖性前列腺癌PC-3细胞中miRNA-21和PTEN的mRNA表和蛋白质表达水平。在前列腺癌PC-3细胞中通过转染miRNA-21inhibitor成功抑制MiRNA-21表达后,实时荧光定量PCR和Westernblot检测PC-3细胞中PTEN表达水平。结果 miRNA-21在前列腺癌组织中的相对表达量为(0.421±0.166),明显高于正常前列腺组织(0.033±0.018)(t=6.6,P﹤0.05)。miRNA-21inhibitor转染PC3细胞后miRNA-21在空白组、转染组和阴性对照组的相对表达量分别为(0.516±0.213)、(0.117±0.044)、(0.392±0.171),转染组与空白组及阴性对照组比较差异有统学意义(F=13.1,q=7.05、4.89,P﹤0.05)。PTENmR-NA在正常前列腺组织、前列腺癌组织的相对表达量分别为(1.534±0.385)、(0.376±0.232),正常前列腺组织高于前列腺癌组织差异有统计学意义(t=5.8,P﹤0.05);PTEN蛋白在正常前列腺组织、前列腺癌组织中的相对表达量分别为(2.010±0.386)和(0.571±0.143),差异有统计学意义(t=12.3,P﹤0.05)。miRNA-21inhibitor转染PC-3细胞后,PTENmRNA在空白组、转染组和阴性对照组组织中的表达差异不显著,但其蛋白的相对表达量分别为(0.399±0.185)、(0.826±0.197)、(0.442±0.149),转染组与空白组及阴性对照组比较差异有统学意义(F=13.9,q=4.79、4.2,P﹤0.05)。结论前列腺癌组织中miRNA-21的表达上调,在转录后水平抑制PTEN表达,miRNA-21有可能成为前列腺癌治疗的新靶点。OBJECTIVE To investigate the expression and regulatory role of microRNA-21 （miR-21） on PTEN and its role in prostate cancer. METHODS miR-21 and PTEN mRNA expression in normal tissue, prostate cancer tissue and prostate cancer cells （PC3） were tested by quantitative real-time PCR. PTEN protein expression was tested by western blot （WB） .Successful- ly transfected with miRNA inhibition MiR-21 expression in PC-3 cells of prostate cancer, real-time PCR and Western blot, the level of expression of PTEN in PC-3 cells, and then the expression changes of PTEN mRNA and protein were tested by PCR and WB. RESULTS miR-21 was up-regulated by 11.7 multiples in prostate cancer compared to normal prostate tissues. Where- as the expression of PTEN mRNA and protein decreased in prostate cancer tissues compared with normal prostate tissues. It prompted that the miR-21 and PTEN was negativeiy correlative. NO obvious difference was found among PTEN mRNA after miR-21 inhibior transfection in the organization of the blank group, transfection group and negative control group, where its relative protein expression levels had obvious difference. CONCLUSION The PTEN abnormal low-expression may paly a key role in prostate cancer due to the the miR-21 up-regulation.

关 键 词：前列腺癌 MIRNA-21 PTEN 

分 类 号：R737.25[医药卫生—肿瘤]