人参皂苷Rd对中国仓鼠肺细胞染色体畸变作用  

Effect of Ginsenoside Rd on Chromosome Aberration in Chinese Hamster Lung Cells

作　　者：高梅 曹冲 朱春花 曲保恩 

机构地区：[1]山东省医药工业研究所,山东省化学药物重点实验室,山东济南250101

出　　处：《中国药物警戒》2013年第3期133-136,共4页Chinese Journal of Pharmacovigilance

摘　　要：目的研究人参皂苷Rd致中国仓鼠肺细胞(Chinesehamster lungcells,CHL)染色体畸变的作用。方法细胞计数法测定人参皂苷Rd对CHL细胞的半数抑制浓度(IC50),根据IC50设立不同剂量组,进行染色体畸变试验,分别观察人参皂苷Rd染毒6、24h及加S9后染毒6h CHL细胞染色体的数目及结构变化,进行染色体畸变分析。结果人参皂苷Rd染毒6、24h及加S9后染毒6h CHL细胞染色体畸变为阴性。结论在本试验条件下,人参皂苷Rd不能引起CHL细胞染色体产生畸变。Objective To explore the effect of ginsenoside Rd on chromosome aberration in Chinese hamster lung cells（CHL）. Methods We used the method of cell counting to determine the IC50 of ginsenoside Rd on CHL cells, then to establish the range of doses according to the ICs0 and to do the cell chromosome aberration experiment. When the CHL cells were exposured to ginsenoside 1Kd at 6h and 24h and plused $9 mixture at 6h respectively, we observed the changes of chromosome number and structure, then to judge the chromosome aberration results. Results Negative response was found at 6h and 24h after the treatment with ginsenoside Rd and at 6h after the addition of S9 mixture. Conclusion Under the condition of this experiment, ginsenoside Rd does not induce chromosome aberration in CHL cells.

关 键 词：人参皂苷RD 中国仓鼠肺细胞 染色体畸变 

分 类 号：R285.5[医药卫生—中药学]