VEGF-D及MMP-12蛋白的表达与非小细胞肺癌纵隔淋巴结转移的相关性研究  

Correlation research between expression of VEGF-D and MMP-12 with mediastinal lymph node metastasis in non-small cell lung cancer

作　　者：梁任技[1] 王元星[1] 韦兵[1] 贺大璞[1] 莫靓[1] 雷正文[1] 

机构地区：[1]南华大学附属第一医院心胸血管外科,湖南衡阳421001

出　　处：《现代医药卫生》2013年第5期641-643,共3页Journal of Modern Medicine & Health

基　　金：衡阳市科技发展计划资助项目[2009(KJ)38]

摘　　要：目的通过对血管内皮细胞生长因子-D(VEGF-D)及基质金属蛋白酶-12(MMP-12)在非小细胞肺癌(NSCLC)组织及癌旁组织中的表达特点进行检测,探讨VEGF-D及MMP-12 2种蛋白的表达与NSCLC纵隔淋巴结转移的相关性。方法采集60例NSCLC组织及其癌旁组织,应用免疫组织化学方法检测VEGF-D及MMP-12蛋白在这2种组织中的表达情况,分析其与临床病理因素之间的关系,以及与纵隔淋巴结转移的相关性。结果 VEGF-D及MMP-12蛋白在NSCLC组织中呈高表达[均为93.3%(56/60)],显著高于癌旁组织[51.7%(31/60),63.3%(38/60)](P<0.01)。在有纵隔淋巴结转移的癌组织中的表达显著高于无纵隔淋巴结转移的癌组织(P<0.05)。在NSCLC组织中,2种蛋白的高表达与性别、年龄、分化程度以及组织学类型无关(P>0.05),但与肿瘤病理学分期有关(P<0.05)。在有纵隔淋巴结转移的癌组织中,VEGF-D与MMP-12蛋白的表达存在相关性(c=0.274,P=0.041)。结论 VEGF-D及MMP-12蛋白在NSCLC组织及癌旁组织中的高表达现象与NSCLC的淋巴结转移密切相关,抑制VEGF-D及MMP-12蛋白的表达有可能为NSCLC的治疗提供新的治疗靶点。Objective To detect the expressions of vascular endothelial growth factor D （VEGF-D） and matrix metallo- proteinases 12 （MMP-12） in the tissues of nonusmall cell lung cancer and para-carcinoma tissue and to investigate their correlation with mediastinal lymph node metastasis （MLNM） of NSCLC. Methods Expressions of VEGF-D and MMP-12 in 60 tissue sam- ples collected from non-small cell lung cancer and peritumoral were detected by immunohistochemistry and their correlations with clinicopathological findings and MLNM were analyzed. Results The VEGF-D and MMP-12 proteins displayed the high expres- sion in the NSCLC tissue[all 93.3%（56/60）],which was significantly higher than[51.7% （31/60） ,63.3%（38/60）] in the MLNM tissue （P〈0.01 ）, and which in the cancer tissues with MLNM were significantly higher than those in that without mediastinal lymph node metastasis of cancer tissues （P〈0.05）.The VEGF-D and MMP- 12 proteins were not correlated with gender, age, degree of dif- ferentiation and histological types （P〉0.05）, but correlated significantly with the stage of PTNM （P〈0.05）. Significant correlations between VEGF-D and MMP-12（c=0.274,P=0.041） were observed in MLNM cancer tissues. Conclusion There is close correla- tion between the higll expression of VEGF-D and MMP-12 in tissues of NSCLC and para-carcinom with MLNM. Inhibiting the ex- pression of VEGF-D and MMP-12 proteins might provide a new target sites in the clinical treatment of NSCLC.

关 键 词：血管内皮生长因子D 基质金属蛋白酶类 癌 非小细胞肺 淋巴转移 纵隔肿瘤 继发性 

分 类 号：R737.9[医药卫生—肿瘤]