分子印迹固相萃取-高效液相色谱法测定食品中苏丹红Ⅰ-Ⅳ  

HPLC Determination of SudanⅠ-Ⅳ in Food with Molecularly Imprinted Solid Phase Extraction

作　　者：徐小艳[1] 邓世俊[1] 孙远明[1] 

机构地区：[1]华南农业大学食品质量与安全研究所广东省食品质量安全重点实验室,广州510642

出　　处：《理化检验（化学分册）》2013年第2期153-155,160,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：广东高校优秀青年创新人才培育项目(209030)

摘　　要：以苏丹红Ⅰ为模板分子,甲基丙烯酸为功能单体合成了苏丹红分子印迹聚合物,作为固相萃取吸附剂,用于食品中苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的测定。样品经乙腈提取,所得提取液分子印迹固相萃取柱,然后用乙醇-正己烷(1+1)溶液作洗脱剂将苏丹红洗下,收集洗出液供高效液相色谱测定。结果表明:聚合物对印迹分子具有很好的亲和性和特异选择性。方法的线性范围为0.5～15 mg·L^(-1),检出限(3S/N)为4μg·kg^(-1)。以辣椒粉和辣椒酱为基体,在3个浓度水平下做加标回收试验,回收率在70.3%～85.5%之间,测定值的相对标准偏差(n=5)低于6.0%。The molecularly imprinted polymer of Sudan red was synthesized with Sudan Ⅰ as template molecule and methacrylic acid as functional monomer, and it was used to extract Sudan Ⅰ-Ⅳ from food as adsorbent in solid phase extraction column. The sample was extracted with acetonitrile, and the extract obtained were purified on MIP-SPE column. Sudan Ⅰ-Ⅳadsorbed on the imprinted polymer were eluted with ethanol-n-hexane （1＋1） mixture, which were collected and used for HPLC determination. It was found that the imprinted polymer had good affinity and excellent selectivity for Sudan Ⅰ-Ⅳ. Linearity range for the determination was found between 0. 5-15 mg·L^-1 , and detection limit （3S/N） was 4μg·kg^-1. Using chili powder and chili sauce as matrixes, test for recovery was made at 3 concentration levels, values of recovery found were in the range of 70. 3%-85. 5% with RSD＇s （n=5） less than 6.0%.

关 键 词：高效液相色谱法 苏丹红Ⅰ-Ⅳ 分子印迹聚合物 固相萃取 

分 类 号：O657.7[理学—分析化学]