高脂血症脂肪肝家兔肝脏纤溶酶原激活物抑制物1型基因表达的研究  

Hepatic plasminogen activator inhibitor type 1 gene expression in rabbit with hyperlipidemia and fatty liver

作　　者：范建高[1] 陈良华[2] 曾民德[3] 王国良[1] 徐正婕[1] 巫协宁[1] 

机构地区：[1]上海市第一人民医院消化科,200080 [2]山东省立医院心内科 [3]上海市消化疾病研究所

出　　处：《中华消化杂志》2000年第4期246-248,共3页Chinese Journal of Digestion

基　　金：国家自然科学基金资助!(39800051)

摘　　要：目的　探讨高脂血症脂肪肝肝组织纤溶酶原激活物抑制物 1型 (PAI 1)mRNA的表达以及血浆PAI 1活性变化。方法　通过持续 12周的高脂饮食建立家兔高脂血症脂肪肝模型 ,分别利用发色底物法和RT PCR法测定模型组 (n =10 )血浆PAI 1活性及肝脏PAI 1mRNA的表达 ,并设正常饮食组 (n =7)作对照。结果　与正常组相比 ,模型组家兔血浆脂质和PAI 1活性在实验 6周时即显著增高 ,实验结束时模型组肝脏呈重度肝细胞脂肪变性 ,肝组织PAI 1mRNA表达较正常组显著增强。结论　高脂血症脂肪肝家兔肝脏PAI 1基因表达增强 ,且可能是高PAIObjective To study the PAI 1 gene expression of hepatic tissue and plasma PAI 1 activity in hyperlipidemia and fatty liver. Methods Rabbit model of hyperlipidemia and fatty liver were induced by high fat diet for 12 weeks, the plasma PAI 1 activity and hepatic PAI 1 mRNA expression were measured ( n =10) with colorimetry and RT PCR, another 7 rabbits were taken as normal control. Results Plasma PAI 1 activity increased in model group at 6 and 12 weeks, and was significantly higher than normal group at 12 weeks. PAI 1 mRNA relative values of hepatic tissue were also higher than normal group (3.474±0.051 vs 1.210±0.031, P <0.01). Conclusion Hepatic PAI 1 gene expression of rabbit with hyperlipidemia and fatty liver was increased, and could be the main cause of high plasma PAI 1.

关 键 词：高脂血症 脂肪肝 PAI-1 基因表达 

分 类 号：R575.5[医药卫生—消化系统]