头颈部鳞癌端粒酶活性的定量检测  

Quantitative assay of telomerase activity in head and neck squamous cell carcinoma

作　　者：张松志[1] 董明敏[2] 滕学敬 陈铁河[3] 张松[1] 

机构地区：[1]新乡医学院第三附属医院耳鼻咽喉科,河南新乡453003 [2]河南医科大学第一附属医院耳鼻咽喉科 [3]上海海军医学研究所

出　　处：《临床耳鼻咽喉科杂志》2000年第9期393-395,共3页Journal of Clinical Otorhinolaryngology

摘　　要：目的 :了解头颈部鳞癌及其颈淋巴结转移癌端粒酶的表达情况 ,探讨端粒酶活性定量分析在头颈部鳞癌诊断中的价值。方法 :采用端粒重复序列液体闪烁计数法检测端粒酶活性。共检测取自 2 5例头颈部鳞癌患者的组织样本 5 5份 ,其中 7例患者同时取有原发癌及其颈淋巴结转移癌两份样本 ,以 2 3份正常组织作为对照。结果 :132份原发鳞癌组织中端粒酶活性 (cpm值 )在 10 0 0以上的 2 8份 ,除 2份外 ,均明显高于正常组织 ;2 3份正常组织的端粒酶活性均在 10 0 0以下 ,除 1份外均低于所有鳞癌组织 (P <0 .0 5 )。 2 7份颈淋巴结转移癌与其原发癌组织同样具有较高的端粒酶活性 (P >0 .0 5 )。结论 :1液体闪烁定量分析方法检测头颈部鳞癌端粒酶活性敏感性较高 ,对头颈部恶性肿瘤的辅助诊断具有一定价值。 2转移癌细胞同样具有较高的端粒酶活性。Objective:To detect and quantify telomerase activity in tissues from head and neck squamous cell carcinoma (HNSCC),and to explore whether the levels of telomerase activity can be a useful marker for cancer risk assessment in HNSCC. Method:The levels of telomerase activity of 55 fresh specimens obtained from 25 patients with HNSCC were detected by the method of telomeric repeat scintillation radioactive count, including 25 primary tumors, 7 samples of neck metastases and 23 corresponding normal tissues as control. In 7 patients, both primary and metastatic tumors were obtained. The levels of telomerase activity were determined with a liquid scintillation counter. The basic principle of the technique is that a G rich oligonucleotide strand of telomeric sequence is used as primer, 3H dTTPs are incorporated into the products while telomerase elongates the primers, and then free 3H dTTPs are removed by rinsing step, finally radioactive count per minute (cpm) of products is detected, and the levels telomerase activity can be evaluated according to the value (cpm). Result:①The levels of telomerase activity (cpm) in tissues with HNSCC were significantly higher than those in the normal tissues (P< 0.05 ); ②The levels of telomerase activity in the primary tumors were higher than those of their neck metastases, but the difference was not statistical significance (P> 0.05 ); ③The levels of telomerase activity in the neck metastases were remarkably higher than those in the normal tissues (P< 0.05 ).Conclusion:①Detection of telomerase activity in HNSCCs can be a useful marker for cancer assessment. ②To quantify telomerase activity may have clinical diagnostic value for HNSCC. ③The levels of telomerase activity in the metastatic lymph nodes were higher.

关 键 词：端粒酶 头颈部肿瘤 鳞状细胞癌 TRAP 定量检测 

分 类 号：R739.91[医药卫生—肿瘤]