1-(3-Aminopropyl)-3-butylimidazolium bromide for carboxyl group derivatization:potential applications in high sensitivity peptide identification by mass spectrometry  

作　　者：QIAO XiaoQiang ZHOU Yuan HOU ChunYan ZHANG XiaoDan YANG KaiGuang ZHANG LiHua ZHANG YuKui 

机构地区：[1]Key Laboratory of Separation Science for Analytical Chemistry,National Chromatographic Research & Analysis Center,Dalian Institute of Chemical Physics,Chinese Academy of Sciences [2]Key Laboratory of Pharmaceutical Quality Control of Hebei Province & College of Pharmaceutical Sciences,Hebei University

出　　处：《Science China(Life Sciences)》2013年第3期240-245,共6页中国科学（生命科学英文版）

基　　金：supported by National Basic Research Program of China (2012CB910604);National Natural Science Foundation of China (21205027,21005079,20935004);Analytical Method Innovation Program of Ministry of Science and Technology of China (2010IM030500);Natural Science Foundation of Hebei Province (B2012201095)

摘　　要：The cationic reagent 1-(3-aminopropyl)-3-butylimidazolium bromide(BAPI) was exploited for the derivatization of carboxyl groups on peptides.Nearly 100% derivatization efficiency was achieved with the synthetic peptide RVYVHPI(RI-7).Furthermore,the peptide derivative was stable in a 0.1% TFA/water solution or a 0.1%(v/v) TFA/acetonitrile/water solution for at least one week.The effect of BAPI derivatization on the ionization of the peptide RI-7 was further investigated,and the detection sensitivity was improved >42-fold via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS),thus outperforming the commercial piperazine derivatization approach.Moreover,the charge states of the peptide were largely increased via BAPI derivatization by electrospray ionization(ESI) MS.The results indicate the potential merits of BAPI derivatization for high sensitivity peptide analysis by MS.The cationic reagent 1-（3-aminopropyl）-3-butylimidazolium bromide （BAPI） was exploited for the derivatization of carboxyl groups on peptides. Nearly 100% derivatization efficiency was achieved with the synthetic peptide RVYVHPI （RI-7）. Furthermore, the pep- tide derivative was stable in a 0.1% TFA/water solution or a 0.1% （v/v） TFA/acetonitrile/water solution for at least one week. The effect of BAPI derivatization on the ionization of the peptide RI-7 was further investigated, and the detection sensitivity was im- proved 〉42-fold via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF MS）, thus outper- forming the commercial piperazine derivatization approach. Moreover, the charge states of the peptide were largely increased via BAPI derivatization by electrospray ionization （ESI） MS. The results indicate the potential merits of BAPI derivatization for high sensitivity peptide analysis by MS.

关 键 词：1-（3-aminopropyl）-3-butylimidazolium bromide ionization capacity peptide mass spectrometry analysis DERIVATIZATION 

分 类 号：Q51[生物学—生物化学]