MCF-7/PTX多药耐药细胞株中CD_(44)^+CD_(24)^-乳腺癌干细胞富集的研究  

Research on the enrichment of CD_(44)^+CD_(24)^- cancer stem cells in breast cancer multi-drug resistant cell lines MCF-7/PTX

作　　者：韩娜娜[1] 孙长岗[1] 庄静[1] 杨静[1] 

机构地区：[1]潍坊市中医院,山东潍坊261041

出　　处：《山东医药》2013年第6期12-14,共3页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(99982679-6106-0)

摘　　要：目的建立人乳腺癌紫杉醇(PTX)多药耐药细胞株MCF-7/PTX,观察MCF-7、MCF-7/PTX细胞株中CD4+4CD2-4乳腺癌干细胞的富集情况。方法以MCF-7为亲本细胞,以PTX为诱导药物,高浓度间歇诱导法建立多药耐药细胞株MCF-7/PTX;MTT法检测MCF-7、MCF-7/PTX对PTX等多种化疗药物的耐药性及交叉耐药性;流式细胞术检测细胞株CD4+4CD2-4乳腺癌干细胞的含量。结果①MCF-7/PTX对PTX、阿霉素、多西紫杉醇、表阿霉素、长春新碱、甲氨蝶呤、顺铂的耐药指数分别为53.00、45.72、38.30、41.26、23.75、17.62、35.80。②MCF-7、MCF-7/PTX细胞株中CD4+4CD2-4细胞的比例分别为8.60%、73.20%,P<0.01。结论本实验所建立的MCF-7/PTX为多药耐药细胞株;MCF-7/PTX存在着CD4+4CD2-4乳腺癌干细胞的富集。Objective To establish human breast cancer multi-drug resistant cell lines MCF-7/PTX in order to study the enrichment of CD＋44 CD-24 breast cancer cells. Methods We established the multidrug resistant cell line MCF-7/PTX by method of intermittently induction with high dose of PTX. The resistance and cross-resistance of MCF-7 and MCF-7/PTX on PTX were identified by MTY method. The proportion of CD＋44 CD-24 cells was detected by method of flow cytometry. Results The resistance indices of MCF-7/PTX on paclitaxel, doxorubicin, docetaxel, epirubicin, vincristine, methotrexate, cis- platin were 53,45.72, 38.30, 41.26, 23.75, 17.62 and 35.80, respectively. The proportion of CD＋44 CD-24 cells in MCF- 7 and MCF-7/PTX were 8.60%, 73.20% , respectively. Conclusion The MCF-7/PTX cell line established in this study was a kind of multi-drug resistant cell line. The CD＋44 CD-24 ceils in MCF-7/PTX were enriched.

关 键 词：肿瘤干细胞 多药耐药 紫杉醇多药耐药细胞株 乳腺癌 

分 类 号：R73-3[医药卫生—肿瘤]