去甲斑蝥素对肝癌细胞AIF蛋白的影响  

Influence of NCTD on AIF protein in liver cancer

作　　者：李先茜[1] 余琛[1] 吴嘉熙[1] 李水军[1] 李晓丽[1] 

机构地区：[1]上海市徐汇区中心医院中心实验室,上海200031

出　　处：《山东医药》2013年第10期7-9,共3页Shandong Medical Journal

基　　金：上海市徐汇区科研基金项目(SHXH201108);上海市徐汇区中心医院科研基金项目(2012XHCH06)

摘　　要：目的探讨去甲斑蝥素(NCTD)对肝癌细胞AIF蛋白的影响。方法向人肝癌细胞SMMC-7721中加入0、10、50、100 g/mL NCTD,应用蛋白质抽提、Western blot方法分析AIF蛋白的表达。结果 0、10、50、100 g/mLNCTD作用于人肝癌细胞SMMC-7721,从线粒体释放入胞质的AIF被活化,AIF的表达量分别为(0.28±1.5)%、(0.37±1.6)%、(0.46±2.2)%,与对照组的(0.14±1.2)%相比,均有统计学差异(P<0.05或<0.01),且其活化程度呈浓度依赖性。结论 AIF蛋白可能作为肝癌治疗过程中判断疗效的分子标志物之一。Objective To investigate the influence of norcantharidin（NCTD） on the AIF protein in hepatoma ceils. Methods The expression of the AIF protein was measured by putting NCTD at different doses（0,10,50,100 g/mL） into the human hepotoma cell SMMC-7721, protein extraction and Western blot method. Results The activities of AIF protein were up-regulated after NCTD at different doses （0,10,50,100 g/mL）. The AIF protein was activated from the mitochon- dria into the cytoplasm. Compared with the control group（0.14±1.2）%, the expression levels were （0.28 ±1.5）%, （0.37±1.6） % and （0.46± 2.2） % in sequence. There were statistical differences between them（ P 〈0. 05 or 〈 0.01）. The degree of activation depended on the concentration of AIF protein. Conclusion AIF protein might be one of molecular markers in liver cancer treatment process.

关 键 词：肝肿瘤 肝癌 药物治疗 细胞凋亡诱导因子蛋白 去甲斑蝥素 

分 类 号：R73[医药卫生—肿瘤]