大鼠脊髓前角与盆神经节之间植块联合培养的实验研究  

Experimental study on co-culture of ventral spinal cord explants and major pelvic ganglion explants

作　　者：程时刚[1] 耿红琼[1] 张伊凡[1] 杨星海[1] 钟伟[2] 

机构地区：[1]湖北省妇幼保健院外科,湖北武汉430070 [2]湖北省肿瘤医院乳腺科,湖北武汉430079

出　　处：《南京医科大学学报（自然科学版）》2013年第2期177-181,共5页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:联合培养大鼠脊髓前角与盆神经节(major pelvic ganglia,MPG)植块并初步探讨两者相互作用的可能性。方法:建立大鼠脊髓前角与盆神经节之间植块的联合培养体系,观察神经元的生长变化。结果:神经组织在体外联合培养时均生长良好,脊髓前角植块生长出的运动神经元(spinal motor neurons,SMNs)发出突起与盆神经节的神经元形成明显形态上的接触。结论:联合培养的不同系统的神经元能彼此形成明显接触,初步提示运动神经元与盆神经节神经元之间可能形成功能性的相互作用。Objective：To co-culture the ventral spinal cord explants and major pelvic ganglion explants from rats and investigate the interaction between co-cultured explants. Methods：We had developed a system for long-term co-culturing of ventral spinal cord explants and major pelvic ganglia explants to observe the variance of neuron growth. Results： Explanted tissues of both types survived well in co-culture. Spinal motor neurons （SMNs） emitted numerous outgrowing processes,some of which associated with neurons in explanted major pelvic ganglia. Conclusion：Some contacts of specific motor neuron-major pelvic ganglia neuron were observed apparently, suggesting that a functional interaction may develop between the spinal motor neurons and major pelvic ganglion neurons in vitro.

关 键 词：脊髓前角 盆神经节 植块 联合培养 

分 类 号：Q421[生物学—神经生物学]